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Université de Neuchâtel

Regeneration of a Lytic Central Vacuole and of Neutral Peripheral Vacuoles Can Be Visualized by Green Fluorescent Proteins Targeted to Either Type of Vacuoles

Di Sansebastiano, Gian Pietro ; Paris, Nadine ; Marc-Martin, Sophie ; Neuhaus, Jean-Marc

In: Plant Physiology, 2001, vol. 126, p. 78-86

Protein trafficking to two different types of vacuoles was investigated in tobacco (Nicotiana tabacum cv SR1) mesophyll protoplasts using two different vacuolar green fluorescent proteins (GFPs). One GFP is targeted to a pH-neutral vacuole by the C-terminal vacuolar sorting determinant of tobacco chitinase A, whereas the other GFP is targeted to an acidic lytic vacuole by the N-terminal...

Université de Neuchâtel

Demonstration in Yeast of the Function of BP-80, a Putative Plant Vacuolar Sorting Receptor

Humair, David ; Hernández Felipe, Doramys ; Neuhaus, Jean-Marc ; Paris, Nadine

In: Plant Cell, 2001, vol. 13, p. 781-792

BP-80, later renamed VSRPS-1, is a putative receptor involved in sorting proteins such as proaleurain to the lytic vacuole, with its N-terminal domain recognizing the vacuolar sorting determinant. Although all VSRPS-1 characteristics and in vitro binding properties described so far favored its receptor function, this function remained to be demonstrated. Here, we used green...

Université de Neuchâtel

The Internal Propeptide of the Ricin Precursor Carries a Sequence-Specific Determinant for Vacuolar Sorting

Frigerio, Lorenzo ; Jolliffe, Nicholas A. ; Di Cola, Alessandra ; Hernández Felipe, Doramys ; Paris, Nadine ; Neuhaus, Jean-Marc ; Lord, J. Michael ; Ceriotti, Aldo ; Roberts, Lynne M.

In: Plant Physiology, 2001, vol. 126, p. 167-175

Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linker is removed. Here, we replicate these events in transfected tobacco (Nicotiana...

Université de Neuchâtel

ArabidopsisµA-adaptin interacts with the tyrosine motif of the vacuolar sorting receptor VSR-PS1

Happel, Nicole ; Höning, Stefan ; Neuhaus, Jean-Marc ; Paris, Nadine ; Robinson, David G. ; Holstein, Suzanne E. H.

In: The Plant Journal, 2004, vol. 37, p. 678-693

In receptor-mediated transport pathways in mammalian cells, clathrin-coated vesicle (CCV) µ-adaptins are the main binding partners for the tyrosine sorting/internalization motif (YXXØ). We have analyzed the function of the µA-adaptin, one of the five µ-adaptins from Arabidopsis thaliana, by pull-down assays and plasmon resonance measurements using its receptor-binding domain (RBD) fused to a...

Université de Neuchâtel

The C-terminal tetrapeptide of phaseolin is sufficient to target green fluorescent protein to the vacuole

Frigerio, Lorenzo ; Foresti, Ombretta ; Hernández Felipe, Doramys ; Neuhaus, Jean-Marc ; Vitale, Alessandro

In: Journal of Plant Physiology, 2001, vol. 158, p. 499-503

Phaseolin is a vacuolar storage glycoprotein synthesized as a precursor with a short C-terminal propeptide. We have recently shown that deletion of the last four C-terminal amino acids (AFVY, which are part of, or constitute the propeptide) abolishes vacuolar targeting, causing phaseolin to be secreted. Here we provide biochemical and microscopical evidence that the AFVY tetrapeptide, when fused...

Université de Neuchâtel

Evolution of the marine stable carbon-isotope record during the early Cretaceous : A focus on the late Hauterivian and Barremian in the Tethyan realm

Godet, Alexis ; Bodin, Stéphane ; Föllmi, Karl B. ; Vermeulen, Jean ; Gardin, Silvia ; Fiet, Nicolas ; Adatte, Thierry ; Berner, Zsolt ; Stüben, Doris ; Van de Schootbrugge, Bas

In: Earth and Planetary Science Letters, 2006, vol. 242, p. 254-271

In order to improve our understanding of the relationships between the late Hauterivian oceanic anoxic Faraoni event, contemporaneous platform drowning along the northern Tethyan margin and global environmental change in general, we established high-resolution δ13C and δ18O curves for the late Hauterivian and the entire Barremian stage. These data were obtained from whole-rock carbonate samples...

Université de Neuchâtel

Algorithms and VLSI architectures for low-power mobile face verification

Nagel, Jean-Luc ; Pellandini, Fausto (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2005 ; 1850.

Parmi les modalités biométriques convenant à la vérification d'identité sur un dispositif portable, la reconnaissance de visage est particulièrement intéressante de par sa faible invasivité, et de par sa simplicité d'usage (du moins du point de vue de l'utilisateur). De plus, le coût du dispositif de vérification d'identité peut être réduit lorsque le senseur d'image est partagé...