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Université de Fribourg

Cross-border emergence of Escherichia coli producing the carbapenemase NDM-5 in Switzerland and Germany

Chakraborty, Trinad ; Sadek, Mustafa ; Yao, Yancheng ; Imirzalioglu, Can ; Stephan, Roger ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2020, p. -

A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5- producing E. coli isolates were highly resistant to β-lactams including the novel ß- lactam/ß-lactamase inhibitor combinations (ceftazidime/avibactam, ...

Université de Fribourg

A standard numbering scheme for class c β-lactamases

Mack, Andrew R. ; Barnes, Melissa D. ; Taracila, Magdalena A. ; Hujer, Andrea M. ; Hujer, Kristine M. ; Cabot, Gabriel ; Feldgarden, Michael ; Haft, Daniel H. ; Klimke, William ; Akker, Focco van den ; Vila, Alejandro J. ; Smania, Andrea ; Haider, Shozeb ; Papp-Wallace, Krisztina M. ; Bradford, Patricia A. ; Rossolini, Gian Maria ; Docquier, Jean-Denis ; Frère, Jean-Marie ; Galleni, Moreno ; Hanson, Nancy D. ; Oliver, Antonio ; Plésiatyz, Patrick ; Poirel, Laurent ; Nordmann, Patrice ; Palzkill, Timothy G. ; Jacoby, George A. ; Bush, Karen ; Bonomo, Robert A.

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 3, p. -

Unlike for classes A and B, a standardized amino acid numbering scheme has not been proposed for the class C (AmpC) β-lactamases, which complicates communication in the field. Here, we propose a scheme developed through a collaborative approach that considers both sequence and structure, preserves traditional numbering of catalytically important residues (Ser64, Lys67, Tyr150, and Lys315),...

Université de Fribourg

Rapid ESBL NP test for rapid detection of expanded-spectrum β-lactamase producers in enterobacterales

Demord, Anthony ; Poirel, Laurent ; D'Emidio, Fernando ; Pomponio, Stefano ; Nordmann, Patrice

In: Microbial Drug Resistance, 2020, p. -

Objectives: To evaluate the biochemical Rapid ESBL NP® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.Methods: A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 ...

Université de Fribourg

Genetic features leading to reduced susceptibility to aztreonam-avibactam among metallo-β-lactamase-producing escherichia coli isolates

Sadek, Mustafa ; Juhas, Mario ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 2020-12, p. -

Metallo-β-lactamase (MBL)-producing Escherichia coli isolates resistant to the newly developed β-lactam/β-lactamase inhibitor drug combination aztreonam-avibactam (ATM-AVI) have been reported. Here, we analyzed a series of 118 clinical MBL- producing E. coli isolates of various geographical origins for susceptibility to ATM-AVI. The nature of the PBP3 protein sequence and the occurrence of...

Université de Fribourg

Dimercaptosuccinic acid in combination with carbapenems against isogenic strains of Escherichia coli producing or not producing a metallo-β-lactamase in vitro and in murine peritonitis

Cheminet, G. ; de Lastours, V. ; Poirel, Laurent ; Chau, F. ; Peoc’h, K. ; Massias, L. ; Fantin, B. ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2020, vol. 75, no. 12, p. 3593–3600

Background: Carbapenemase-producing Enterobacterales represent a major therapeutic challenge. MBLs, requiring zinc at their catalytic site, could be inhibited by meso-dimercaptosuccinic acid (DMSA), a heavy metal chelator already widely used for treating lead intoxication.Objectives: To evaluate the activity of carbapenems alone or combined with DMSA against MBL-producing Escherichia coli in...

Université de Fribourg

Cutaneous inputs to dorsal column nuclei in adult macaque monkeys subjected to unilateral lesion of the primary motor cortex or of the cervical spinal cord and treatments promoting axonal growth

Savidan, Julie ; Beaud, Marie-Laure ; Rouiller, Eric M.

In: Neuroscience Insights, 2020, vol. 15, p. 2633105520973991

The highly interconnected somatosensory and motor systems are subjected to connectivity changes at close or remote locations following a central nervous system injury. What is the impact of unilateral injury of the primary motor cortex (hand area; MCI) or of the cervical cord (hemisection at C7-C8 level; SCI) on the primary somatosensory (cutaneous) inputs to the dorsal column nuclei (DCN) in...

Université de Fribourg

Optogenetic stimulation of basal forebrain parvalbumin neurons activates the default mode network and associated behaviors

Lozano-Montes, Laura ; Dimanico, Marta ; Mazloum, Reza ; Li, Wenxue ; Nair, Jayakrishnan ; Kintscher, Michael ; Schneggenburger, Ralf ; Harvey, Michael ; Rainer, Gregor

In: Cell Reports, 2020, vol. 33, no. 6, p. 108359

Activation of the basal forebrain (BF) has been associated with increased attention, arousal, and a heightened cortical representation of the external world. In addition, BF has been implicated in the regulation of the default mode network (DMN) and associated behaviors. Here, we provide causal evidence for a role of BF in DMN regulation, highlighting a prominent role of parvalbumin (PV)...

Université de Fribourg

Biophysics of temporal interference stimulation

Mirzakhalili, Ehsan ; Barra, Beatrice ; Capogrosso, Marco ; Lempka, Scott F.

In: Cell Systems, 2020, vol. 11, no. 6, p. 557-572.e5

Temporal interference (TI) is a non-invasive neurostimulation technique that utilizes high-frequency external electric fields to stimulate deep neuronal structures without affecting superficial, off-target structures. TI represents a potential breakthrough for treating conditions, such as Parkinson’s disease and chronic pain. However, early clinical work on TI stimulation was met with mixed...

Université de Fribourg

Epilepsy-associated neurocognitive disorders (EAND) in an onchocerciasis-endemic rural community in Cameroon : A population-based case–control study

K.Njamnshi, Alfred ; Chokote, Eric-Samuel ; Ngarka, Leonard ; Nfor, Leonard N. ; Tabaha, Earnest N. ; Basseguin Atchou, Jonas G. ; Angwaforbe, Samuel A. ; Nkouonlack, Cyrille ; Mengnjo, Michel K. ; Njamnshi, Wepnyu Y. ; Demaa, Fidèle ; Tataha, Godwin Y. ; Zoung-KanyiBissek, Anne-Cecile ; Annoni, Jean-Marie ; Ruffieux, Nicolas

In: Epilepsy & Behavior, 2020, vol. 112, p. 107437

Epilepsy affects at least 50 million individuals worldwide, especially in sub-Saharan Africa (sSA). Cognitive impairment is common in people with epilepsy (PWE) yet, little is known on the burden of cognitive impairment in people with epilepsy in sSA. This study was thus designed to assess cognitive impairment in PWE or epilepsy- associated neurocognitive disorders (EAND) in a rural...

Université de Fribourg

Distal and proximal hypoxia response elements cooperate to regulate organ-specific erythropoietin gene expression

Orlando, Ilaria M. C. ; Lafleur, Véronique N. ; Storti, Federica ; Spielmann, Patrick ; Crowther, Lisa ; Santambrogio, Sara ; Schödel, Johannes ; Hoogewijs, David ; Mole, David R. ; Wenger, Roland H.

In: Haematologica, 2019, vol. 105, no. 12, p. 2774–2784

While it is well-established that distal hypoxia response elements (HREs) regulate hypoxia-inducible factor (HIF) target genes such as erythropoietin (Epo), an interplay between multiple distal and proximal (promoter) HREs has not been described so far. Hepatic Epo expression is regulated by a HRE located downstream of the EPO gene, but this 3' HRE is dispensable for renal EPO gene...