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Accès public à partir du 30 janv. 2020
Università della Svizzera italiana

The contribution of active and passive mechanisms of 5mC and 5hmC removal in human T lymphocytes is differentiation- and activation-dependent

Vincenzetti, Lucia ; Leoni, Cristina ; Chirichella, Michele ; Kwee, Ivo ; Monticelli, Silvia

In: European Journal of Immunology, 2019, p. 1-15

In mammals, the 5’-methylcytosine (5mC) modification in the genomic DNA contributes to the dynamic control of gene expression. 5mC erasure is required for the activation of developmental programs and occurs either by passive dilution through DNA replication, or by enzymatic oxidation of the methyl mark to 5-hydroxymethylcytosine (5hmC), which can persist as such or undergo further oxidation...

Université de Fribourg

Antimicrobial Resistance in Escherichia coli

Poirel, Laurent ; Madec, Jean-Yves ; Lupo, Agnese ; Schink, Anne-Kathrin ; Kieffer, Nicolas ; Nordmann, Patrice ; Schwarz, Stefan

In: Microbiology Spectrum, 2018, vol. 6, no. 4, p. -

Multidrug resistance in Escherichia coli has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. E. coli is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic...

Université de Fribourg

Acquisition of extended-spectrum β-lactamase GES-6 leading to resistance to ceftolozane-tazobactam combination in Pseudomonas aeruginosa

Poirel, Laurent ; Ortiz De La Rosa, José-Manuel ; Kieffer, Nicolas ; Dubois, Véronique ; Jayol, Aurélie ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 1, p. e01809-18

A clinical Pseudomonas aeruginosa isolate resistant to all β-lactams, including ceftolozane-tazobactam and carbapenems, was recovered. It belonged to sequence type 235 and produced the extended-spectrum β-lactamase (ESBL) GES-6 differing from GES-1 by two amino acid substitutions (E104K and G170S). GES-6 possessed an increased hydrolytic activity toward carbapenems and to ceftolozane and a ...

Université de Fribourg

T cells loaded with magnetic nanoparticles are retained in peripheral lymph nodes by the application of a magnetic field

Sanz‑Ortega, Laura ; Rojas, José M. ; Marcos, Ana ; Portilla, Yadileiny ; Stein, Jens V. ; Barber, Domingo F.

In: Journal of Nanobiotechnology, 2019, vol. 17, no. 1, p. 14

T lymphocytes are highly dynamic elements of the immune system with a tightly regulated migration. T cell-based transfer therapies are promising therapeutic approaches which in vivo efficacy is often limited by the small proportion of administered cells that reaches the region of interest. Manipulating T cell localisation to improve specific targeting will increase the effectiveness of these...

Université de Fribourg

Aging effects on prefrontal cortex oxygenation in a posture-cognition dual-task: an fNIRS pilot study

Marusic, Uros ; Taube, Wolfgang ; Morrison, Shawnda A. ; Biasutti, Lea ; Grassi, Bruno ; Pauw, Kevin De ; Meeusen, Romain ; Pisot, Rado ; Ruffieux, Jan

In: European Review of Aging and Physical Activity, 2019, vol. 16, no. 1, p. 2

The aging process alters upright posture and locomotion control from an automatically processed to a more cortically controlled one. The present study investigated a postural-cognitive dual-task paradigm in young and older adults using functional Near- Infrared Spectroscopy (fNIRS).Methods: Twenty healthy participants (10 older adults 72 ± 3 y, 10 young adults 23 ± 3 y) performed a...

Università della Svizzera italiana

A selective ER‐phagy exerts procollagen quality control via a Calnexin‐FAM134B complex

Forrester, Alison ; Leonibus, Chiara De ; Grumati, Paolo ; Fasana, Elisa ; Piemontese, Marilina ; Staiano, Leopoldo ; Fregno, Ilaria ; Raimondi, Andrea ; Marazza, Alessandro ; Bruno, Gemma ; Iavazzo, Maria ; Intartaglia, Daniela ; Seczynska, Marta ; van Anken, Eelco ; Conte, Ivan ; De Matteis, Maria Antonietta ; Dikic, Ivan ; Molinari, Maurizio ; Settembre, Carmine

In: The EMBO Journal, 2019, vol. 38, no. 2, p. e99847

Autophagy is a cytosolic quality control process that recognizes substrates through receptor‐mediated mechanisms. Procollagens, the most abundant gene products in Metazoa, are synthesized in the endoplasmic reticulum (ER), and a fraction that fails to attain the native structure is cleared by autophagy. However, how autophagy selectively recognizes misfolded procollagens in the ER lumen is...

Università della Svizzera italiana

ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens

Proietti, Michele ; Perruzza, Lisa ; Scribano, Daniela ; Pellegrini, Giovanni ; D’Antuono, Rocco ; Strati, Francesco ; Raffaelli, Marco ; Gonzalez, Santiago F. ; Thelen, Marcus ; Hardt, Wolf-Dietrich ; Slack, Emma ; Nicoletti, Mauro ; Grassi, Fabio

In: Nature Communications, 2019, vol. 10, p. Art. number: 250

T cell dependent secretory IgA (SIgA) generated in the Peyer’s patches (PPs) of the small intestine shapes a broadly diverse microbiota that is crucial for host physiology. The mutualistic co-evolution of host and microbes led to the relative tolerance of host’s immune system towards commensal microorganisms. The ATP-gated ionotropic P2X7 receptor limits T follicular helper (Tfh) cells...

Università della Svizzera italiana

The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection

Levikova, Maryna ; Pinto, Cosimo ; Cejka, Petr

In: Genes and Development, 2017, vol. 31, no. 5, p. 493-502

DNA2 nuclease–helicase functions in DNA replication and recombination. This requires the nuclease of DNA2, while, in contrast, the role of the helicase activity has been unclear. We now show that the motor activity of both recombinant yeast and human DNA2 promotes efficient degradation of long stretches of ssDNA, particularly in the presence of the replication protein A. This degradation is...

Université de Fribourg

H2S, a Bacterial Defense Mechanism against the Host Immune Response

Toliver-Kinsky, Tracy ; Cui, Weihua ; Törö, Gabor ; Lee, Seung-Jin ; Shatalin, Konstantin ; Nudler, Evgeny ; Szabo, Csaba

In: Infection and Immunity, 2019, vol. 87, no. 1, p. e00272-18

The biological mediator hydrogen sulfide (H2S) is produced by bacteria and has been shown to be cytoprotective against oxidative stress and to increase the sensitivity of various bacteria to a range of antibiotic drugs. Here we evaluated whether bacterial H2S provides resistance against the immune response, using two bacterial species that are common sources of nosocomial infections,...

Université de Fribourg

Cystathionine γ Lyase Sulfhydrates the RNA Binding Protein Human Antigen R to Preserve Endothelial Cell Function and Delay Atherogenesis

Bibli, Sofia-Iris ; Hu, Jiong ; Sigala, Fragiska ; Wittig, Ilka ; Heidler, Juliana ; Zukunft, Sven ; Tsilimigras, Diamantis I. ; Randriamboavonjy, Voahanginirina ; Wittig, Janina ; Kojonazarov, Baktybek ; Schürmann, Christoph ; Siragusa, Mauro ; Siuda, Daniel ; Luck, Bert ; Malik, Randa Abdel ; Filis, Konstantinos A. ; Zografos, George ; Chen, Chen ; Wang, Dao Wen ; Pfeilschifter, Josef ; Brandes, Ralf P. ; Szabo, Csaba ; Papapetropoulos, Andreas ; Fleming, Ingrid

In: Circulation, 2019, vol. 139, no. 1, p. 101–114

Hydrogen sulfide (H2S), generated by cystathionine γ lyase (CSE), is an important endogenous regulator of vascular function. The aim of the present study was to investigate the control and consequences of CSE activity in endothelial cells under physiological and proatherogenic conditions.