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Université de Neuchâtel

The Tick Blood Meal: From a Living Animalor from a Silicone Membrane ?

Kröber, Thomas ; Guerin, Patrick M.

In: Altex, 2007, vol. 24, p. 39-41

An artificial feeding unit with a reinforced silicone membrane to replace host skin provides ticks with a perch over blood with a tick attachment rate of 75-100%. Some 5 mg of an acaricide like fipronil is sufficient to establish survival curves over different doses down to ppb levels in blood. This in vitro feeding assay for hard ticks is more advantageous than in vivotrials on animals.

Université de Neuchâtel

Effect of mating frequency and brood cell infestation rate on the reproductive success of the honeybee parasite Varroa jacobsoni

Donzé, Gérard ; Herrmann, Miriam ; Bachofen, Boris ; Guerin, Patrick M.

In: Physiological Entomology, 1996, vol. 21, no. 1, p. 17-26

1. The reproductve biology of Varroa jacobsoni, whose females infest honeybee brood, was studied in natural and transparent artificial brood cells. These investigations were made under the headings of maturation behaviour and fertilization, and the influence of infestation rate of brood cells on the number of mated females produced per infesting Varroa. 2. Mating of Varroa daughters,...

Université de Neuchâtel

The life-cycle of the bont tick Amblyomma hebraeum in vitro

Kuhnert, Frank ; Diehl, Peter A. ; Guerin, Patrick M.

In: International Journal for Parasitology, 1995, vol. 25, no. 8, p. 887-896

The life-cycle of the hard tick Amblyomma hebraeum was completed in vitro by feeding all life-stages of the tick through silicone membranes on bovine blood from an abattoir. Ticks were placed in a simple feeder consisting of a honey jar containing the blood with a glass tube insert (o.d. 42 mm) across the end of which the membrane was stretched. This feeding unit was held in a water...