Université de Fribourg

The transillumination possibility of imidazole–osmium postfixed tissue and its consequences for the handling of tissue samples

Voigt, Tilman ; Dauber, Wolfgang

In: Microscopy and Microanalysis, 2005, vol. 11(1), p. 42

Osmium postfixation is established as a routine procedure for transmission electron microscopy (TEM). On the one hand, this routine procedure leads to good results for TEM, but on the other hand results in blackened tissue samples that do not allow examination of any structures within the embedded tissue sample by a light microscope. Equivalent fixation results for TEM are achieved with...

Université de Fribourg

The transillumination possibility of imidazole-osmium postfixed muscle tissue and its consequences for the handling of muscle tissue samples

Voigt, Tilman ; Dauber, Wolfgang

In: Microscopy Research and Technique, 2004, vol. 63(2), p. 129

The osmium postfixation of tissue leads to good results for transmission electron microscopy, but also produces completely blackened tissue samples that do not allow the recognition of internal structures. With imidazole-osmium postfixation, one achieves comparable results in high electron microscopic resolution as with routine osmium postfixation. But the tissue samples are not blackened and...