Université de Neuchâtel

Optimisation and comparison of transient expression methods to express the green fluorescent protein in the obligate biotrophic oomycete Plasmopara viticola

Dubresson, Romain ; Kravchuk, Z. ; Neuhaus, Jean-Marc ; Mauch-Mani, Brigitte

In: Vitis, 2008, vol. 47, no. 4, p. 235-240

Grape downy mildew is caused by Plasmopara viti-cola, an obligate biotrophic oomycete and a major path-ogen of grapevine. Studying obligate biotrophic patho-gens is difficult as they cannot grow without their host. We therefore attempted to develop a method where the pathogen could be visualized and quantified in planta without killing the host plant. To this end P. viticola was...

Université de Neuchâtel

β-Aminobutyric Acid-Induced Resistance Against Downy Mildew in Grapevine Acts Through the Potentiation of Callose Formation and Jasmonic Acid Signaling

Hamiduzzaman, Mollah Md. ; Jakab, Gabor ; Barnavon, Laurent ; Neuhaus, Jean-Marc ; Mauch-Mani, Brigitte

In: Molecular Plant Microbe-Interactions, 2005, vol. 18, no. 8, p. 819-829

β-Aminobutyric acid (BABA) was used to induce resistance in grapevine (Vitis vinifera) against downy mildew (Plasmopara viticola). This led to a strong reduction of mycelial growth and sporulation in the susceptible cv. Chasselas. Comparing different inducers, the best protection was achieved with BABA followed by jasmonic acid (JA), whereas benzo (1,2,3)-thiadiazole-7-carbothionic...