Université de Neuchâtel

Inhibition Studies of Porphobilinogen Synthase from Escherichia coli Differentiating between the Two Recognition Si

Stauffer, Frédéric ; Zizzari, Eleonora ; Engeloch-Jarret, Caroline ; Faurite, Jean-Philippe ; Bobálová, Janette ; Neier, Reinhard

In: ChemBioChem, 2001, vol. 2, no. 5, p. 343-354

Porphobilinogen synthase condenses two molecules of 5-aminolevulinate in an asymmetric way. This unusual transformation requires a selective recognition and differentiation between the substrates ending up in the A site or in the P site of porphobilinogen synthase. Studies of inhibitors based on the key intermediate first postulated by Jordan allowed differentiation of the two recognition sites....