Université de Fribourg

An analog to digital converter controls bistable transfer competence development of a widespread bacterial integrative and conjugative element

Carraro, Nicolas ; Richard, Xavier ; Sulser, Sandra ; Delavat, Franc¸ois ; Mazza, Christian ; van der Meer, Jan Roelof

In: eLife, 2020, vol. 9, p. e57915

Conjugative transfer of the integrative and conjugative element ICEclc in Pseudomonas requires development of a transfer competence state in stationary phase, which arises only in 3–5% of individual cells. The mechanisms controlling this bistable switch between non-active and transfer competent cells have long remained enigmatic. Using a variety of genetic tools and epistasis experiments in...

Haute Ecole d'Ingénierie

Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

Truffer, Frederic ; Buffi, Nina ; Merulla, Davide ; Beggah, Siham ; van Lintel, Harald ; Renaud, Philippe ; van der Meer, Jan Roelof ; Geiser, Martial

In: Review of Scientific Instruments, 2014, vol. 85, no. 015120, p. 4

We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated...