Université de Neuchâtel

Plastid Proteome Assembly without Toc159: Photosynthetic Protein Import and Accumulation of N-Acetylated Plastid Precursor Proteins

Bischof, Sylvain ; Baerenfaller, Katja ; Wildhaber, Thomas ; Troesch, Raphael ; Vidi, Pierre-Alexandre ; Roschitzki, Bernd ; Hirsch-Hoffmann, Matthias ; Hennig, Lars ; Kessler, Felix ; Gruissem, Wilhelm ; Baginsky, Sacha

In: The Plant Cell, 2011, vol. 23, no. 11, p. 3911-3928

Import of nuclear-encoded precursor proteins from the cytosol is an essential step in chloroplast biogenesis that is mediated by protein translocon complexes at the inner and outer envelope membrane (TOC). Toc159 is thought to be the main receptor for photosynthetic proteins, but lacking a large-scale systems approach, this hypothesis has only been tested for a handful of photosynthetic and...

Université de Neuchâtel

Plastoglobules Are Lipoprotein Subcompartments of the Chloroplast That Are Permanently Coupled to Thylakoid Membranes and Contain Biosynthetic Enzymes

Austin, Jotham R. ; Frost, Elizabeth ; Vidi, Pierre-Alexandre ; Kessler, Felix ; Staehelin, L. Andrew

In: The Plant Cell, 2006, vol. 18, no. 7, p. 1693-1703

Plastoglobules are lipoprotein particles inside chloroplasts. Their numbers have been shown to increase during the upregulation of plastid lipid metabolism in response to oxidative stress and during senescence. In this study, we used state-of-the-art high-pressure freezing/freeze-substitution methods combined with electron tomography as well as freeze-etch electron microscopy to characterize the...

Université de Neuchâtel

Intersection of the tocopherol and plastoquinol metabolic pathways at the plastoglobule

Zbierzak, Anna Maria ; Kanwischer, Marion ; Wille, Christina ; Vidi, Pierre-Alexandre ; Giavalisco, Patrick ; Lohmann, Antje ; Briesen, Isabel ; Porfirova, Svetlana ; Bréhélin, Claire ; Kessler, Felix ; Dörmann, Peter

In: Biochemical Journal, 2010, vol. 425, p. 389-399

Plastoglobules, lipid–protein bodies in the stroma of plant chloroplasts, are enriched in non-polar lipids, in particular prenyl quinols. In the present study we show that, in addition to the thylakoids, plastoglobules also contain a considerable proportion of the plastidial PQ-9 (plastoquinol-9), the redox component of photosystem II, and of the cyclized product of PQ-9, PC-8...

Université de Neuchâtel

A mutation in the Arabidopsis mTERF-related plastid protein SOLDAT10 activates retrograde signaling and suppresses 1O2-induced cell death

Meskauskiene, Rasa ; Würsch, Marco ; Laloi, Christophe ; Vidi, Pierre-Alexandre ; Coll, Núria S. ; Kessler, Felix ; Baruah, Aiswarya ; Kim, Chanhong ; Apel, Klaus

In: The Plant Journal, 2009, vol. 60, no. 3, p. 399-410

The conditional flu mutant of Arabidopsis thaliana generates singlet oxygen (1O2) in plastids during a dark-to-light shift. Seedlings of flu bleach and die, whereas mature plants stop growing and develop macroscopic necrotic lesions. Several suppressor mutants, dubbed singlet oxygen-linked death activator (soldat), were identified that abrogate...

Université de Neuchâtel

Plastoglobules: a new address for targeting recombinant proteins in the chloroplast

Vidi, Pierre-Alexandre ; Bréhélin, Claire ; Kessler, Felix

In: BMC Biotechnology, 2007, vol. 7, no. 4, p. 1-12

Background The potential of transgenic plants for cost-effective production of pharmaceutical molecules is now becoming apparent. Plants have the advantage over established fermentation systems (bacterial, yeast or animal cell cultures) to circumvent the risk of pathogen contamination, to be amenable to large scaling up and to necessitate only established farming procedures....

Université de Neuchâtel

On the role of chloroplast plastoglobule lipoprotein particles vitamin E biosynthesis

Vidi, Pierre-Alexandre ; Kessler, Felix (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2006 ; 1881.

Les chloroplastes contiennent des corps protéo-lipidiques connus sous le nom de plastoglobules. Les plastoglobules ont longtemps été considérés comme des sites de stockage de lipides. Cependant, des études ont montré que leur nombre et leur volume augmente dans des situations de stress oxydatif. Ces structures ont donc un comportement dynamique. Des protéines structurelles appartenant à...

Université de Neuchâtel

Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles

Vidi, Pierre-Alexandre ; Kanwischer, Marion ; Baginsky, Sacha ; Austin, Jotham R. ; Csucs, Gabor ; Dörmann, Peter ; Kessler, Felix ; Bréhélin, Claire

In: The Journal of Biological Chemistry, 2006, vol. 281, p. 11225-11234

Chloroplasts contain lipoprotein particles termed plastoglobules. Plastoglobules are generally believed to have little function beyond lipid storage. Here we report on the identification of plastoglobule proteins using mass spectrometry methods in Arabidopsis thaliana. We demonstrate specific plastoglobule association of members of the plastid lipid-associated proteins/fibrillin family as...