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Université de Fribourg

The innate immune signaling system as a regulator of disease resistance and induced systemic resistance activity against Verticillium dahliae

Gkizi, Danai ; Lehmann, Silke ; L’Haridon, Floriane ; Serrano, Mario ; Paplomatas, Epaminondas J. ; Métraux, Jean-Pierre ; Tjamos, Sotirios E.

In: Molecular Plant-Microbe Interactions, 2016, vol. 29, no. 4, p. 313–323

In the last decades, the plant innate immune responses against pathogens have been extensively studied, while biocontrol interactions between soilborne fungal pathogens and their hosts have received much less attention. Treatment of Arabidopsis thaliana with the nonpathogenic bacterium Paenibacillus alvei K165 was shown previously to protect against Verticillium dahliae by triggering induced...

Université de Fribourg

The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana

Hael-Conrad, V. ; Abou-Mansour, Eliane ; Díaz-Ricci, J.-C. ; Métraux, Jean-Pierre ; Serrano, Mario

In: Plant Science, 2015, vol. 241, p. 120–127

AsES (Acremonium strictum Elicitor and Subtilisin) is a novel extracellular elicitor protein produced by the avirulent isolate SS71 of the opportunist strawberry fungal pathogen A. strictum. Here we describe the activity of AsES in the plant-pathogen system Arabidopsis thaliana–Botrytis cinerea. We show that AsES renders A. thaliana plants resistant to the necrotrophic pathogen B. cinerea, both...

Université de Fribourg

Considerations for designing chemical screening strategies in plant biology

Serrano, Mario ; Kombrink, Erich ; Meesters, Christian

In: Frontiers in Plant Science - Plant Physiology, 2015, vol. 6, p. 131

Traditionally, biologists regularly used classical genetic approaches to characterize and dissect plant processes. However, this strategy is often impaired by redundancy, lethality or pleiotropy of gene functions, which prevent the isolation of viable mutants. The chemical genetic approach has been recognized as an alternative experimental strategy, which has the potential to circumvent these...

Université de Fribourg

Reactive oxygen species and plant resistance to fungal pathogens

Lehmann, Silke ; Serrano, Mario ; L’Haridon, Floriane ; Tjamos, Sotirios E. ; Metraux, Jean-Pierre

In: Phytochemistry, 2015, vol. 112, p. 54–62

Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the...

Université de Fribourg

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

Abou-Mansour, Eliane ; Débieux, Jean-Luc ; Ramírez-Suero, Montserrat ; Bénard-Gellon, Mélanie ; Magnin-Robert, Maryline ; Spagnolo, Alessandro ; Chong, Julie ; Farine, Sibylle ; Bertsch, Christohpe ; L’Haridon, Floriane ; Serrano, Mario ; Fontaine, Florence ; Rego, Cecilia ; Larignon, Philippe

In: Phytochemistry, 2015, vol. 115, p. 207–215

Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic...

Université de Fribourg

The cuticle and plant defense to pathogens

Metraux, Jean-Pierre ; Serrano, Mario ; Torres, Martha ; Coluccia, Fania ; L’Haridon, Floriane

In: Frontiers in Plant Science, 2014, vol. 5, p. 274

The cuticle provides a physical barrier against water loss and protects against irradiation, xenobiotics and pathogens. Components of the cuticle are perceived by invading fungi and activate developmental processes during pathogenesis. In addition, cuticle alterations of various types induce a syndrome of reactions that often results in resistance to necrotrophs. This article reviews the current...

Université de Fribourg

Expression of a microbial serine proteinase inhibitor gene enhances the tobacco defense against oomycete pathogens

Silva, Yussuan ; Portieles, Roxana ; Pujol, Merardo ; Terauchi, Ryohei ; Matsumura, Hideo ; Serrano, Mario ; Borrás-Hidalgo, Orlando

In: Physiological and Molecular Plant Pathology, 2013, vol. 84, p. 99–106

In order to identify Nicotiana megalosiphon genes for novel inhibitors of microbial serine proteinase involved in resistance to the oomycete Phytophthora parasitica var. nicotianae, SuperSAGE technology combined with next-generation sequencing were used to generate libraries in order to identify transcripts that are differentially up-regulated. We identified a N. megalosiphon inhibitor of...

Université de Fribourg

Perception of soft mechanical stress in Arabidopsis leaves activates disease resistance

Benikhlef, Lehcen ; L’Haridon, Floriane ; Abou-Mansour, Eliane ; Serrano, Mario ; Binda, Matteo ; Costa, Alex ; Lehmann, Silke ; Métraux, Jean-Pierre

In: BMC Plant Biology, 2013, vol. 13, no. 1, p. 133

Background: in a previous study we have shown that wounding of Arabidopsis thaliana leaves induces a strong and transient immunity to Botrytis cinerea, the causal agent of grey mould. Reactive oxygen species (ROS) are formed within minutes after wounding and are required for wound–induced resistance to B. cinerea.Results: in this study, we have further explored ROS and resistance to B. cinerea...

Université de Fribourg

export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-Like transporter EDS5

Serrano, Mario ; Wang, Bangjun ; Aryal, Bibek ; Garcion, Christophe ; Abou-Mansour, Eliane ; Heck, Silvia ; Geisler, Markus ; Mauch, Felix ; Nawrath, Christiane ; Métraux, Jean-Pierre

In: Plant Physiology, 2013, vol. 162, no. 4, p. 1815–1821

Salicylic acid (SA) is central for the defense of plants to pathogens and abiotic stress. SA is synthesized in chloroplasts from chorismic acid by an isochorismate synthase (ICS1); SA biosynthesis is negatively regulated by autoinhibitory feedback at ICS1. Genetic studies indicated that the multidrug and toxin extrusion transporter ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5) of Arabidopsis...

Université de Fribourg

A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity

L'Haridon, Floriane ; Besson-Bard, Angélique ; Binda, Matteo ; Serrano, Mario ; Abou-Mansour, Eliane ; Balet, Francine ; Schoonbeek, Henk-Jan ; Hess, Stephane ; Mir, Ricardo ; León, José ; Lamotte, Olivier ; Métraux, Jean-Pierre

In: PLoS Pathogens, 2011, vol. 7, no. 7, p. e1002148

This study provides an explanation for the strong resistance to B. cinerea observed in wounded plants or plants with cuticular defects. We have observed that a production of ROS and a permeable cuticle is common to all these situations. ROS, that include hydrogen peroxide, are known inducers of resistance and can also act directly against the invading fungus. Degradation of the cuticle by...