Consortium of Swiss Academic Libraries

Alpha Complementation of LacZ in Mammalian Cells

Moosmann, Peter ; Rusconi, Sandro

In: Nucleic Acids Research, 1996, vol. 24, no. 6, p. 1171-1172

Université de Fribourg

Interaction of circadian clock proteins PER2 and CRY with BMAL1 and CLOCK

Langmesser, Sonja ; Tallone, Tiziano ; Bordon, Alain ; Rusconi, Sandro ; Albrecht, Urs

In: BMC Molecular Biology, 2008, vol. 9, p. 41

Background: Circadian oscillation of clock-controlled gene expression is mainly regulated at the transcriptional level. Heterodimers of CLOCK and BMAL1 act as activators of target gene transcription; however, interactions of PER and CRY proteins with the heterodimer abolish its transcriptional activation capacity. PER and CRY are therefore referred to as negative regulators of the circadian...

Université de Fribourg

A role for skeletal muscle stearoyl-CoA desaturase 1 in control of thermogenesis

Mainieri, Davide ; Summermatter, Serge ; Seydoux, Josiane ; Montani, Jean-Pierre ; Rusconi, Sandro ; Russell, Aaron P. ; Boss, Olivier ; Buchala, Antony J. ; Dulloo, Abdul G.

In: The FASEB Journal, 2006, vol. 20, no. 10, p. 1751-1753

An enhanced metabolic efficiency for accelerating the recovery of fat mass (or catch-up fat) is a characteristic feature of body weight regulation after weight loss or growth retardation and is the outcome of an "adipose-specific" suppression of thermogenesis, i.e., a feedback control system in which signals from the depleted adipose tissue fat stores exert a suppressive effect on thermogenesis....

Université de Fribourg

The direct effect of leptin on skeletal muscle thermogenesis is mediated by substrate cycling between de novo lipogenesis and lipid oxidation

Solinas, Giovanni ; Summermatter, Serge ; Mainieri, Davide ; Gubler, Marcel ; Pirola, Luciano ; Wymann, Matthias P. ; Rusconi, Sandro ; Montani, Jean-Pierre ; Seydoux, Josiane

In: FEBS Letters, 2004, vol. 577 (3), p. 539-544

We report here studies that integrate data of respiration rate from mouse skeletal muscle in response to leptin and pharmacological interference with intermediary metabolism, together with assays for phosphatidylinositol 3-kinase (PI3K) and AMP-activated protein kinase (AMPK). Our results suggest that the direct effect of leptin in stimulating thermogenesis in skeletal muscle is mediated by...

Université de Fribourg

Thrombin Stimulates Human Endothelial Arginase Enzymatic Activity via RhoA/ROCK Pathway

Ming, Xiu-Fen ; Barandier, Christine ; Viswambharan, Hema ; Kwak, Brenda R. ; Mach, François ; Mazzolai, Lucia ; Hayoz, Daniel ; Ruffieux, Jean ; Rusconi, Sandro ; Montani, Jean-Pierre ; Yang, Zhihong

In: Circulation, 2004, vol. 110, p. 3708-3714

Background— Arginase competes with endothelial nitric oxide synthase (eNOS) for the substrate L-arginine and decreases NO production. This study investigated regulatory mechanisms of arginase activity in endothelial cells and its role in atherosclerosis. Methods and Results— In human endothelial cells isolated from umbilical veins, thrombin concentration- and time-dependently stimulated...