Université de Fribourg

In vivo quantification of mechanical properties of caudal fins in adult zebrafish

Puri, Sahil ; Aegerter-Wilmsen, Tinri ; Jaźwińska, Anna ; Aegerter, Christof M.

In: Journal of Experimental Biology, 2018, vol. 221, no. 4, p. jeb171777

Skip to Next SectionThe caudal fins of adult zebrafish are supported by multiple bony rays that are laterally interconnected by soft interray tissue. Little is known about the fin's mechanical properties that influence bending in response to hydrodynamic forces during swimming. Here, we developed an experimental setup to measure the elastic properties of caudal fins in vivo by applying...

Université de Fribourg

Dynamics of actinotrichia regeneration in the adult zebrafish fin

König, Désirée ; Page, Lionel ; Chassot, Bérénice ; Jaźwińska, Anna

In: Developmental Biology, 2018, vol. 433, no. 2, p. 416–432

The skeleton of adult zebrafish fins comprises lepidotrichia, which are dermal bones of the rays, and actinotrichia, which are non-mineralized spicules at the distal margin of the appendage. Little is known about the regenerative dynamics of the actinotrichia- specific structural proteins called Actinodins. Here, we used immunofluorescence analysis to determine the contribution of two...

Université de Fribourg

The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

Pfefferli, Catherine ; Jaźwińska, Anna

In: Nature Communications, 2017, vol. 8, p. 15151

The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Activin-β signalling in the peri-injury zone of the myocardium and the fin mesenchyme. In...

Université de Fribourg

Collagen XII contributes to epicardial and connective tissues in the Zebrafish heart during ontogenesis and regeneration

Marro, Jan ; Pfefferli, Catherine ; Preux Charles, Anne-Sophie de ; Bise, Thomas ; Jaźwińska, Anna

In: PLOS ONE, 2016, vol. 11, no. 10, p. e0165497

Zebrafish heart regeneration depends on cardiac cell proliferation, epicardium activation and transient reparative tissue deposition. The contribution and the regulation of specific collagen types during the regenerative process, however, remain poorly characterized. Here, we identified that the non-fibrillar type XII collagen, which serves as a matrix-bridging component, is expressed in the...

Université de Fribourg

Preconditioning boosts regenerative programmes in the adult zebrafish heart

Preux Charles, Anne-Sophie de ; Bise, Thomas ; Baier, Felix ; Sallin, Pauline ; Jaźwińska, Anna

In: Open Biology, 2016, vol. 6, no. 7, p. 160101

During preconditioning, exposure to a non-lethal harmful stimulus triggers a body-wide increase of survival and pro-regenerative programmes that enable the organism to better withstand the deleterious effects of subsequent injuries. This phenomenon has first been described in the mammalian heart, where it leads to a reduction of infarct size and limits the dysfunction of the injured organ....

Université de Fribourg

Distinct effects of inflammation on preconditioning and regeneration of the adult zebrafish heart

Preux Charles, Anne-Sophie de ; Bise, Thomas ; Baier, Felix ; Marro, Jan ; Jaźwińska, Anna

In: Open Biology, 2016, vol. 6, no. 7, p. 160102

The adult heart is able to activate cardioprotective programmes and modifies its architecture in response to physiological or pathological changes. While mammalian cardiac remodelling often involves hypertrophic expansion, the adult zebrafish heart exploits hyperplastic growth. This capacity depends on the responsiveness of zebrafish cardiomyocytes to mitogenic signals throughout their entire...

Université de Fribourg

Zebrafish fin regeneration after cryoinjury-induced tissue damage

Chassot, Bérénice ; Pury, David ; Jaźwińska, Anna

In: Biology Open, 2016, vol. 5, no. 6, p. 819–828

Although fin regeneration following an amputation procedure has been well characterized, little is known about the impact of prolonged tissue damage on the execution of the regenerative programme in the zebrafish appendages. To induce histolytic processes in the caudal fin, we developed a new cryolesion model that combines the detrimental effects of freezing/thawing and ischemia. In contrast...

Université de Fribourg

Acute stress is detrimental to heart regeneration in zebrafish

Sallin, Pauline ; Jaźwińska, Anna

In: Open Biology, 2016, vol. 6, no. 3, p. 160012

Psychological stress is one of the factors associated with human cardiovascular disease. Here, we demonstrate that acute perceived stress impairs the natural capacity of heart regeneration in zebrafish. Beside physical and chemical disturbances, intermittent crowding triggered an increase in cortisol secretion and blocked the replacement of fibrotic tissue with new myocardium. Pharmacological...

Université de Fribourg

Regeneration versus scarring in vertebrate appendages and heart

Jaźwińska, Anna ; Sallin, Pauline

In: The Journal of Pathology, 2015, p. -

Injuries to complex human organs, such as the limbs and the heart, result in pathological conditions, for which we often lack adequate treatments. While modern regenerative approaches are based on the transplantation of stem cell-derived cells, natural regeneration in lower vertebrates, such as zebrafish and newts, relies predominantly on the intrinsic plasticity of mature tissues. This property...

Université de Fribourg

Bone morphogenetic protein signaling promotes morphogenesis of blood vessels, wound epidermis, and actinotrichia during fin regeneration in zebrafish

Thorimbert, Valentine ; König, Désirée ; Marro, Jan ; Ruggiero, Florence ; Jaźwińska, Anna

In: The FASEB Journal, 2015, p. fj.15–272955

Zebrafish fin regeneration involves initial formation of the wound epidermis and the blastema, followed by tissue morphogenesis. The mechanisms coordinating differentiation of distinct tissues of the regenerate are poorly understood. Here, we applied pharmacologic and transgenic approaches to address the role of bone morphogenetic protein (BMP) signaling during fin restoration. To map the BMP...