Université de Neuchâtel

Development of an Array of Ion-Selective Microelectrodes Aimed for the Monitoring of Extracellular Ionic Activities

Guenat, Olivier T. ; Generelli, Silvia ; de Rooij, Nicolaas F. ; Koudelka-Hep, Milena ; Berthiaume, François ; Yarmush, Martin L.

In: Analytical Chemistry, 2006, vol. 78, no. 21, p. 7453–7460

In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4+) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or differentiation. The platform for cell culture is equipped with an array of 16 silicon nitride micropipet-based...

Université de Neuchâtel

Ion-selective microelectrode arrays for cell culture monitoring

Generelli, Silvia ; De Rooij, Nicolas-F. (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2008 ; Th. 2042.

The design, microfabrication and characterization of a platform comprising an array of ion-selective microelectrodes (µISE) aimed at in vitro cellular physiology and toxicology is described. This study focusses on K+ and Ca2+ monitoring in cell culture environments. A potential promising application of such a platform is based on recent findings in molecular biology,...

Université de Neuchâtel

Generic technological platform for microfabricating silicon nitride micro- and nanopipette arrays

Guenat, Olivier T. ; Generelli, Silvia ; Dadras, Mohammad-Mehdi ; Berdondini, L. ; de Rooij, Nicolaas F. ; Koudelka-Hep, Milena

In: Journal of Micromechanics and Microengineering, 2005, vol. 15, no. 12, p. 2372-2378

In this paper, the design and the characterization of batch fabricated SixNy micropipette arrays with diameters ranging from 6 µm down to 250 nm are described. The process used to fabricate the micromachined pipettes includes a deep reactive ion etching step, followed by the deposition of two successive layers, a thermal oxide layer and a low stress, low pressure chemical...

Université de Neuchâtel

Microfabricated platforms for the study of neuronal and cellular networks

Berdondini, L. ; Generelli, Silvia ; Kraus, T. ; Guenat, Olivier T. ; Koster, S. ; Linder. V. ; Koudelka-Hep, Milena ; de Rooij, Nicolaas F.

In: Journal of Physics : Conference Series, 2006, vol. 34, no. 1, p. 1-6

In this contribution we present the development of three microfabricated devices for the study of neuronal and cellular networks. Together, these devices form an attractive toolbox, which is useful to stimulate and record signals of both electrical and chemical nature. One approach consist of microelectrode arrays for the study of neuronal networks, and allow for the electrical stimulation of...