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Université de Fribourg

The impact of ESCRT on Aβ1-42 induced membrane lesions in a yeast model for Alzheimer’s disease

Fruhmann, Gernot ; Marchal, Christelle ; Vignaud, Hélène ; Verduyckt, Mathias ; Talarek, Nicolas ; De Virgilio, Claudio ; Winderickx, Joris ; Cullin, Christophe

In: Frontiers in Molecular Neuroscience, 2018, vol. 11, p. -

Aβ metabolism plays a pivotal role in Alzheimer’s disease. Here, we used a yeast model to monitor Aβ42 toxicity when entering the secretory pathway and demonstrate that processing in, and exit from the endoplasmic reticulum (ER) is required to unleash the full Aβ42 toxic potential. Consistent with previously reported data, our data suggests that Aβ42 interacts with mitochondria, thereby...

Université de Fribourg

The architecture of the Rag GTPase signaling network

Nicastro, Raffaele ; Sardu, Alessandro ; Panchaud, Nicolas ; De Virgilio, Claudio

In: Biomolecules, 2017, vol. 7, no. 3, p. 48

The evolutionarily conserved target of rapamycin complex 1 (TORC1) couples an array of intra- and extracellular stimuli to cell growth, proliferation and metabolism, and its deregulation is associated with various human pathologies such as immunodeficiency, epilepsy, and cancer. Among the diverse stimuli impinging on TORC1, amino acids represent essential input signals, but how they control...

Consortium of Swiss Academic Libraries

The essence of yeast quiescence

De Virgilio, Claudio

In: FEMS Microbiology Reviews, 2012, vol. 36, no. 2, p. 306-339

Université de Fribourg

The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis

Numrich, Johannes ; Péli-Gulli, Marie-Pierre ; Arlt, Henning ; Sardu, Alessandro ; Griffith, Janice ; Levine, Tim ; Engelbrecht-Vandré, Siegfried ; Reggiori, Fulvio ; De Virgilio, Claudio ; Ungermann, Christian

In: Journal of Cell Science, 2015, vol. 128, no. 13, p. 2278–2292

Membrane fusion at the vacuole depends on a conserved machinery that includes SNAREs, the Rab7 homolog Ypt7 and its effector HOPS. Here, we demonstrate that Ypt7 has an unexpected additional function by controlling membrane homeostasis and nutrient-dependent signaling on the vacuole surface. We show that Ivy1, the yeast homolog of mammalian missing-in-metastasis (MIM), is a vacuolar effector of...

Université de Fribourg

TORC1 controls G1-S cell cycle transition in yeast via Mpk1 and the greatwall kinase pathway

Moreno-Torres, Marta ; Jaquenoud, Malika ; De Virgilio, Claudio

In: Nature Communications, 2015, vol. 6, p. 8256

The target of rapamycin complex 1 (TORC1) pathway couples nutrient, energy and hormonal signals with eukaryotic cell growth and division. In yeast, TORC1 coordinates growth with G₁–S cell cycle progression, also coined as START, by favouring the expression of G₁ cyclins that activate cyclin-dependent protein kinases (CDKs) and by destabilizing the CDK inhibitor Sic1. Following TORC1...

Université de Fribourg

Quantification of mRNA stability of stress-responsive yeast genes following conditional excision of open reading frames

Talarek, Nicolas ; Bontron, Séverine ; De Virgilio, Claudio

In: RNA Biology, 2013, vol. 10, no. 8, p. 1299–1306

Eukaryotic cells rapidly adjust the levels of mRNAs in response to environmental stress primarily by controlling transcription and mRNA turnover. How different stress conditions influence the fate of stress-responsive mRNAs, however, is relatively poorly understood. This is largely due to the fact that mRNA half-life assays are traditionally based on interventions (e.g., temperature-shifts using...

Université de Fribourg

Amino acid deprivation inhibits TORC1 through a GTPase-activating protein complex for the rag family GTPase Gtr1

Panchaud, Nicolas ; Péli-Gulli, Marie-Pierre ; De Virgilio, Claudio

In: Science Signaling, 2013, vol. 6, no. 277, p. ra42

The Rag family of guanosine triphosphatases (GTPases) regulates eukaryotic cell growth in response to amino acids by activating the target of rapamycin complex 1 (TORC1). In humans, this pathway is often deregulated in cancer. In yeast, amino acids promote binding of GTP (guanosine 5'-triphosphate) to the Rag family GTPase Gtr1, which, in combination with a GDP (guanosine diphosphate)–bound...

Université de Fribourg

Yeast endosulfines control entry into quiescence and chronological life span by inhibiting protein phosphatase 2A

Bontron, Séverine ; Jaquenoud, Malika ; Vaga, Stefania ; Talarek, Nicolas ; Bodenmiller, Bernd ; Aebersold, Ruedi ; De Virgilio, Claudio

In: Cell Reports, 2012, vol. 3, no. 1, p. 16–22

The TORC1 and PKA protein kinases are central elements of signaling networks that regulate eukaryotic cell proliferation in response to growth factors and/or nutrients. In yeast, attenuation of signaling by these kinases following nitrogen and/or carbon limitation activates the protein kinase Rim15, which orchestrates the initiation of a reversible cellular quiescence program to ensure normal...