Université de Neuchâtel

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Paris, Nadine ; Saint-Jean, Bruno ; Faraco, Marianna ; Krzeszowiec, Weronika ; Dalessandro, Giuseppe ; Neuhaus, Jean-Marc ; Di Sansebastiano, Gian Pietro

In: PLANT CELL REPORTS, 2010, vol. 29, no. 1, p. 79-86

The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an α-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a β-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing...

Université de Neuchâtel

Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins

Flückiger, Ricardo ; De Caroli, Monica ; Piro, Gabriella ; Dalessandro, Giuseppe ; Neuhaus, Jean-Marc ; Di Sansebastiano, Gian-Pietro

In: Journal of Experimental Botany, 2003, vol. 54, p. 1577-1584

Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two...