Université de Fribourg

Dynamics of actinotrichia regeneration in the adult zebrafish fin

König, Désirée ; Page, Lionel ; Chassot, Bérénice ; Jaźwińska, Anna

In: Developmental Biology, 2018, vol. 433, no. 2, p. 416–432

The skeleton of adult zebrafish fins comprises lepidotrichia, which are dermal bones of the rays, and actinotrichia, which are non-mineralized spicules at the distal margin of the appendage. Little is known about the regenerative dynamics of the actinotrichia- specific structural proteins called Actinodins. Here, we used immunofluorescence analysis to determine the contribution of two...

Université de Fribourg

Zebrafish fin regeneration after cryoinjury-induced tissue damage

Chassot, Bérénice ; Pury, David ; Jaźwińska, Anna

In: Biology Open, 2016, vol. 5, no. 6, p. 819–828

Although fin regeneration following an amputation procedure has been well characterized, little is known about the impact of prolonged tissue damage on the execution of the regenerative programme in the zebrafish appendages. To induce histolytic processes in the caudal fin, we developed a new cryolesion model that combines the detrimental effects of freezing/thawing and ischemia. In contrast...

Université de Fribourg

Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae

Rohwedder, Astrid ; Selcho, Mareike ; Chassot, Bérénice ; Thum, Andreas S.

In: Journal of Comparative Neurology, 2015, vol. 523, no. 18, p. 2637–2664

All organisms continuously have to adapt their behavior according to changes in the environment in order to survive. Experience-driven changes in behavior are usually mediated and maintained by modifications in signaling within defined brain circuits. Given the simplicity of the larval brain of Drosophila and its experimental accessibility on the genetic and behavioral level, we analyzed if...

Université de Fribourg

Sequencing and characterizing the genome of Estrella lausannensis as an undergraduate project: training students and biological insights

Bertelli, Claire ; Aeby, Sébastien ; Chassot, Bérénice ; Clulow, James ; Hilfiker, Olivier ; Rappo, Samuel ; Ritzmann, Sébastien ; Schumacher, Paolo ; Terrettaz, Céline ; Benaglio, Paola ; Falquet, Laurent ; Farinelli, Laurent ; Gharib, Walid H. ; Goesmann, Alexander ; Harshman, Keith ; Linke, Burkhard ; Miyazaki, Ryo ; Rivolta, Carlo ; Robinson-Rechavi, Marc ; Meer, Jan Roelof van der ; Greub, Gilbert

In: Evolutionary and Genomic Microbiology, 2015, vol. 6, p. 101

With the widespread availability of high-throughput sequencing technologies, sequencing projects have become pervasive in the molecular life sciences. The huge bulk of data generated daily must be analyzed further by biologists with skills in bioinformatics and by “embedded bioinformaticians,” i.e., bioinformaticians integrated in wet lab research groups. Thus, students interested in...

Université de Fribourg

Early expression of the type III secretion system of Parachlamydia acanthamoebae during a replicative cycle within its natural host cell Acanthamoeba castellanii

Croxatto, Antony ; Murset, Valérie ; Chassot, Bérénice ; Greub, Gilbert

In: Pathogens and Disease, 2013, p. –

The type three secretion system (T3SS) operons of Chlamydiales bacteria are distributed in different clusters along their chromosomes and are conserved at both the level of sequence and genetic organization. A complete characterization of the temporal expression of multiple T3SS components at the transcriptional and protein levels has been performed in Parachlamydia acanthamoebae, replicating in...