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Université de Neuchâtel

Role of Soil Macrofauna in Phosphorus Cycling

Chapuis-Lardy, Lydie ; Le Bayon, Renée-Claire ; Brossard, Michel ; López-Hernández, Danilo ; Blanchart, Eric

In: Phosphorus in Action : Biological Processes in Soil Phosphorus Cycling (Series “Soil Biology”, vol. 26), 2011, vol. 8, p. 199-213

Université de Neuchâtel

Ixodes ricinus L. : mast cells, basophils and eosinophils in the sequence of cellular events in the skin of infested or re-infested rabbits

Brossard, Michel ; Fivaz, V.

In: Parasitology, 1982, vol. 85, no. 3, p. 583-592

Some information on acquired resistance of rabbits against Ixodes ricinus ticks has been obtained by comparing tissue changes and cellular infiltration during 2 successive infestations, with particular emphasis on the cells involved in immediate type hypersensitivity reactions: mast cells, basophils and eosinophils. More degranulated mast cells were observed in tick lesions 2 h after the...

Université de Neuchâtel

Identification of an Ixodes ricinus salivary gland fraction through its ability to stimulate CD4 T cells present in BALB/c mice lymph nodes draining the tick fixation site

Ganapamo, F. ; Rutti, Bernard ; Brossard, Michel

In: Parasitoloty, 1997, vol. 115, no. 1, p. 91-96

BALB/c mice infested with larvae or nymphs of Ixodes ricinus develop in their lymph nodes a T cell-specific immune response triggered by salivary gland soluble antigens (SGA). SGA are apparently conserved in the 3 biological stages of I. ricinus ticks and are species specific. SGA derived from partially fed females I. ricinus stimulate lymph node T cells from mice infested...

Université de Neuchâtel

Progressive sensitization of circulating basophils against Ixodes ricinus L. antigens during repeated infestations of rabbits

Brossard, Michel ; Monneron, J.-P. ; Papatheodorou, V.

In: Parasite Immunology, 1981, vol. 4, no. 5, p. 355-361

The sensitivity of rabbit basophils to antigens from Ixodes ricinus females has been studied by a degranulation test. Observations of basophil numbers and degranulation were made on the 6th day of each of four sequential infestations. Maximal degranulation of cells was observed after challenge of cells with antigen at a concentration of 106 and 107 pg/ml. At these...

Université de Neuchâtel

Th2 polarization of the immune response of BALB/c mice to Ixodes ricinus instars, importance of several antigens in activation of specific Th2 subpopulations

Mejri, Naceur ; Franscini, Nicola ; Rutti, Bernard ; Brossard, Michel

In: Parasite Immunology, 2001, vol. 23, no. 2, p. 61-69

BALB/c mice were infested with Ixodes ricinus larvae, nymphs or adults. Expression of IL-4 and IFN-γ mRNA in axillary and brachial draining lymph node cells were measured by competitive quantitative reverse transcription-polymerase chain reaction 9 days after the beginning of primary-infestation. IL-4 mRNA was always higher than that of IFN-γ mRNA for all tick instars. Moreover, IL-4 mRNA...