Université de Neuchâtel

The Acidic A-Domain of Arabidopsis Toc159 Occurs as a Hyperphosphorylated Protein

Agne, Birgit ; Andrès, Charles ; Montandon, Cyril ; Christ, Bastien ; Ertan, Anouk ; Jung, Friederike ; Infanger, Sibylle ; Bischof, Sylvain ; Baginsky, Sacha ; Kessler, Felix

In: Plant Physiology, 2010, vol. 53, no. 3, p. 1016-1030

The translocon at the outer membrane of the chloroplast assists the import of a large class of preproteins with amino-terminal transit sequences. The preprotein receptors Toc159 and Toc33 in Arabidopsis (Arabidopsis thaliana) are specific for the accumulation of abundant photosynthetic proteins. The receptors are homologous GTPases known to be regulated by phosphorylation within their...

Université de Neuchâtel

The Chloroplast Import Receptor Toc90 Partially Restores the Accumulation of Toc159 Client Proteins in the Arabidopsis thaliana ppi2 Mutant

Infanger, Sibylle ; Bischof, Sylvain ; Hiltbrunner, Andreas ; Agne, Birgit ; Baginsky, Sacha ; Kessler, Felix

In: Molecular Plant, 2011, vol. 4, no. 2, p. 252–263

Successful import of hundreds of nucleus-encoded proteins is essential for chloroplast biogenesis. The import of cytosolic precursor proteins relies on the Toc- (translocon at the outer chloroplast membrane) and Tic- (translocon at the inner chloroplast membrane) complexes. In Arabidopsis thaliana, precursor recognition is mainly mediated by outer membrane receptors belonging to two...

Université de Neuchâtel

Plastid Proteome Assembly without Toc159: Photosynthetic Protein Import and Accumulation of N-Acetylated Plastid Precursor Proteins

Bischof, Sylvain ; Baerenfaller, Katja ; Wildhaber, Thomas ; Troesch, Raphael ; Vidi, Pierre-Alexandre ; Roschitzki, Bernd ; Hirsch-Hoffmann, Matthias ; Hennig, Lars ; Kessler, Felix ; Gruissem, Wilhelm ; Baginsky, Sacha

In: The Plant Cell, 2011, vol. 23, no. 11, p. 3911-3928

Import of nuclear-encoded precursor proteins from the cytosol is an essential step in chloroplast biogenesis that is mediated by protein translocon complexes at the inner and outer envelope membrane (TOC). Toc159 is thought to be the main receptor for photosynthetic proteins, but lacking a large-scale systems approach, this hypothesis has only been tested for a handful of photosynthetic and...

Université de Neuchâtel

In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system

Rahim, Gwendoline ; Bischof, Sylvain ; Kessler, Felix ; Agne, Birgit

In: Journal of Experimental Botany, 2008, vol. 60, no. 1, p. 257-267

The GTPases atToc33 and atToc159 are pre-protein receptor components of the translocon complex at the outer chloroplast membrane in Arabidopsis. Despite their participation in the same complex in vivo;, evidence for their interaction is still lacking. Here, a split-ubiquitin system is engineered for use in plants, and the in vivo; interaction of the Toc GTPases...