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Université de Fribourg

Magi1, a new potential tumor suppressor gene in estrogen receptor positive breast cancer

Alday-Parejo, Begoña ; Richard, François ; Wörthmüller, Janine ; Rau, Tilman ; Galván, José A. ; Desmedt, Christine ; Santamaria-Martinez, Albert ; Rüegg, Curzio

In: Cancers, 2020, vol. 12, no. 1, p. 223

Membrane-associated guanylate kinase (MAGUK) with inverted domain structure-1 (MAGI1) is an intracellular adaptor protein that stabilizes epithelial junctions consistent with a tumor suppressive function in several cancers of epithelial origin. Here we report, based on experimental results and human breast cancer (BC) patients’ gene expression data, that MAGI1 is highly expressed and acts...

Université de Fribourg

Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa ; Tinguely, Camille ; Poirel, Laurent ; Nordmann, Patrice

In: European Journal of Clinical Microbiology & Infectious Diseases, 2020, p. -

Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually...

Université de Fribourg

Occurrence of NDM-1-producing Morganella morganii and Proteus mirabilis in a single patient in Portugal: probable in vivo transfer by conjugation

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Goncalves, Maria Luísa ; Costa, Augusto ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2020, vol. 75, no. 4, p. 903–906

To decipher the genetics of acquisition of carbapenemase-encoding genes identified in two carbapenem-resistant Enterobacteriaceae recovered from a single patient in Portugal.Methods: Carbapenemase genes were searched by PCR assays and mating-out assays were performed to further characterize the plasmid support of the carbapenemase genes. Genetic characterization of the plasmid supports was...

Université de Fribourg

Optimal detection of extended-spectrum β-lactamase producers, carbapenemase producers, polymyxin-resistant Enterobacterales, and vancomycin-resistant enterococci from stools

Sadek, Mustafa ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 96, no. 1, p. 114919

This study compared the impact of different approaches, namely, nonenrichment, nonselective enrichment, and selective (antibiotic-containing) enrichment steps, for detecting extended-spectrum β-lactamase producing Enterobacterales (ESBL-E), carbapenemase-producing Enterobacterales (CPE), polymyxin-resistant Enterobacterales (PMR-E), and vancomycin- resistant enterococci (VRE) from spiked...

Université de Fribourg

ISEcp1 mediated transposition leads to fosfomycin and broad-spectrum cephalosporin resistance in Klebsiella pneumoniae

Kieffer, Nicolas ; Poirel, Laurent ; Mueller, Linda ; Mancini, Stefano ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 5, p. -

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed ISEcp1-blaCTX-M-14b tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the...

Université de Fribourg

Characterization of FosL1, a plasmid-encoded fosfomycin resistance protein identified in Escherichia coli

Kieffer, Nicolas ; Poirel, Laurent ; Descombes, Marie-Christine ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 4, p. -

Fosfomycin is gaining renewed interest for treating urinary tract infections. Monitoring fosfomycin resistance is therefore important in order to detect the emergence of novel resistance mechanisms. Here, we used the Rapid Fosfomycin NP test to screen a collection of extended-spectrum-β-lactamase- producing Escherichia coli isolates from Switzerland and found a fosfomycin- resistant isolate...

Université de Fribourg

γδ T cells kill Plasmodium falciparum in a granzyme- and granulysin-dependent mechanism during the late blood stage

Hernández-Castañeda, Maria Andrea ; Happ, Katharina ; Cattalani, Filippo ; Wallimann, Alexandra ; Blanchard, Marianne ; Fellay, Isabelle ; Scolari, Brigitte ; Lannes, Nils ; Mbagwu, Smart Ikechukwu ; Fellay, Benoît ; Filgueira, Luis ; Mantel, Pierre-Yves ; Walch, Michael

In: The Journal of Immunology, 2020, vol. 204, no. 7, p. 1798–1809

Plasmodium spp., the causative agent of malaria, have a complex life cycle. The exponential growth of the parasites during the blood stage is responsible for almost all malaria-associated morbidity and mortality. Therefore, tight immune control of the intraerythrocytic replication of the parasite is essential to prevent clinical malaria. Despite evidence that the particular lymphocyte subset ...

Université de Fribourg

Postnatal development of the entorhinal cortex: a stereological study in macaque monkeys

Piguet, Olivia ; Chareyron, Loïc J. ; Banta Lavenex, Pamela ; Amaral, David G. ; Lavenex, Pierre

In: Journal of Comparative Neurology, 2020, p. cne.24897

The entorhinal cortex is the main gateway for interactions between the neocortex and the hippocampus. Distinct regions, layers, and cells of the hippocampal formation exhibit different profiles of structural and molecular maturation during postnatal development. Here, we provide estimates of neuron number, neuronal soma size, and volume of the different layers and subdivisions of the monkey...