Université de Neuchâtel

The Chloroplast Import Receptor Toc90 Partially Restores the Accumulation of Toc159 Client Proteins in the Arabidopsis thaliana ppi2 Mutant

Infanger, Sibylle ; Bischof, Sylvain ; Hiltbrunner, Andreas ; Agne, Birgit ; Baginsky, Sacha ; Kessler, Felix

In: Molecular Plant, 2011, vol. 4, no. 2, p. 252–263

Successful import of hundreds of nucleus-encoded proteins is essential for chloroplast biogenesis. The import of cytosolic precursor proteins relies on the Toc- (translocon at the outer chloroplast membrane) and Tic- (translocon at the inner chloroplast membrane) complexes. In Arabidopsis thaliana, precursor recognition is mainly mediated by outer membrane receptors belonging to two...

Université de Neuchâtel

Dimerization of Toc-GTPases at the Chloroplast Protein Import Machinery

Hiltbrunner, Andreas ; Kessler, Felix ; Weibel, Petra

In: The Journal of Biological Chemistry, 2003, vol. 278, p. 37321-37329

Import of chloroplast precursor proteins is controlled by the coordinate action of two homologous GTPases, Toc159 and Toc33, located at the cytosol-outer membrane interface. Recent studies in Arabidopsis showed that the cytosolic form of the precursor binding protein Toc159 is targeted to its receptor at the import machinery, Toc33, via heterodimerization of their GTP-binding domains....

Université de Neuchâtel

AtToc90, a New GTP-Binding Component of the Arabidopsis Chloroplast Protein Import Machinery

Hiltbrunner, Andreas ; Grünig, Kathrin ; Alvarez-Huerta, Mayte ; Infanger, Sibylle ; Bauer, Jörg ; Kessler, Felix

In: Plant Molecular Biology, 2004, vol. 54, p. 427 - 440

AtToc159 is a GTP-binding chloroplast protein import receptor. In vivo, atToc159 is required for massive accumulation of photosynthetic proteins during chloroplast biogenesis. Yet, in mutants lacking atToc159 photosynthetic proteins still accumulate, but at strongly reduced levels whereas non-photosynthetic proteins are imported normally: This suggests a role for the homologues of atToc159...