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Université de Fribourg

The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits

Fernández-Pevida, Antonio ; Martín-Villanueva, Sara ; Murat, Guillaume ; Lacombe, Thierry ; Kressler, Dieter ; Cruz, Jesús de la

In: Nucleic Acids Research, 2016, vol. 44, no. 16, p. 7777–7791

The archaea-/eukaryote-specific 40S-ribosomal-subunit protein S31 is expressed as an ubiquitin fusion protein in eukaryotes and consists of a conserved body and a eukaryote-specific N-terminal extension. In yeast, S31 is a practically essential protein, which is required for cytoplasmic 20S pre-rRNA maturation. Here, we have studied the role of the N-terminal extension of the yeast S31...

Université de Fribourg

Processing of preribosomal RNA in Saccharomyces cerevisiae

Fernández-Pevida, Antonio ; Kressler, Dieter ; Cruz, Jesús de la

In: Wiley Interdisciplinary Reviews: RNA, 2015, vol. 6, no. 2, p. 191–209

Most, if not all RNAs, are transcribed as precursors that require processing to gain functionality. Ribosomal RNAs (rRNA) from all organisms undergo both exo- and endonucleolytic processing. Also, in all organisms, rRNA processing occurs inside large preribosomal particles and is coupled to nucleotide modification, folding of the precursor rRNA (pre-rRNA), and assembly of the ribosomal proteins...

Université de Fribourg

Final Pre-40S Maturation Depends on the Functional Integrity of the 60S Subunit Ribosomal Protein L3

García-Gómez, Juan J. ; Fernández-Pevida, Antonio ; Lebaron, Simon ; Rosado, Iván V. ; Tollervey, David ; Kressler, Dieter ; Cruz, Jesús de la

In: PLoS Genetics, 2014, vol. 10, no. 3, p. e1004205

Ribosomal protein L3 is an evolutionarily conserved protein that participates in the assembly of early pre-60S particles. We report that the rpl3[W255C] allele, which affects the affinity and function of translation elongation factors, impairs cytoplasmic maturation of 20S pre-rRNA. This was not seen for other mutations in or depletion of L3 or other 60S ribosomal proteins. Surprisingly, pre-40S...

Université de Fribourg

Yeast ribosomal protein L40 assembles late into pre-60S ribosomes and is required for their cytoplasmic maturation

Fernandez-Pevida, Antonio ; Rodriguez-Galan, Olga ; Diaz-Quintana, Antonio ; Kressler, Dieter ; Cruz, Jesus de la

In: Journal of Biological Chemistry, 2012, p. -

Most ribosomal proteins play important roles in ribosome biogenesis and function. Herein, we have examined the contribution of the essential ribosomal protein L40 in these processes in the yeast Saccharomyces cerevisiae. Deletion of either the RPL40A or RPL40B gene and in vivo depletion of L40 impair 60S ribosomal subunit biogenesis. Polysome profile analyses reveal the accumulation of half-mers...