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Université de Fribourg

Multicellular human alveolar model composed of epithelial cells and primary immune cells for hazard assessment

Barosova, Hana ; Drasler, Barbara ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: JoVE (Journal of Visualized Experiments), 2020, no. 159, p. e61090

A human alveolar cell coculture model is described here for simulation of the alveolar epithelial tissue barrier composed of alveolar epithelial type II cells and two types of immune cells (i.e., human monocyte-derived macrophages [MDMs] and dendritic cells [MDDCs]). A protocol for assembling the multicellular model is provided. Alveolar epithelial cells (A549 cell line) are grown and...

Université de Fribourg

Taylor dispersion of inorganic nanoparticles and comparison to dynamic light scattering and transmission electron microscopy

A.Urban, Dominic ; M.Milosevic, Ana ; Bossert, David ; Crippa, Federica ; L.Moore, Thomas ; Geers, Christoph ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Colloid and Interface Science Communications, 2018, vol. 22, p. 29–33

Taylor dispersion analysis (TDA) is an analytical method that has so far mainly been utilized to determine the diffusion coefficient of small molecules, and proteins. Due to increasing interest in nanoscience, some research has been done on the applicability of TDA towards characterizing nanoparticles. This work aims to expand this knowledge and give insight into the range for which TDA can...

Université de Fribourg

Light-responsive azo-containing organogels

Ayer, Mathieu A. ; Schrettl , Stephen ; Balog, Sandor ; Simon, Yoan C. ; Weder, Christoph

In: Soft Matter, 2017, vol. 13, no. 22, p. 4017-4023

While azo compounds are widely employed as radical initiators, they have rarely been used as stimuli-responsive motifs in macromolecular constructs. In this study, an azo-based cross-linker was prepared and reacted with poly(vinyl alcohol) to afford a series of stimuli-responsive organogels. Irradiation of these materials with UV light causes de-cross-linking and triggers a solid-to-liquid phase...

Université de Fribourg

Azo-Containing Polymers with Degradation On-Demand Feature

Ayer, Mathieu A. ; Simon, Yoan C. ; Weder, Christoph

In: Macromolecules, 2016, vol. 49, p. 2917–2927

Molecules comprising aliphatic azo moieties are widely used as radical polymerization initiators, but only a few studies have explored their usefulness as stimuli-responsive motifs in macromolecular constructs. The controlled degradation of azo-containing polymers has indeed remained largely unexplored. Here we present the syntheses of linear azo-containing polyamides and polyurethanes and...

Université de Fribourg

Nonthermal Melting of Néel Order in the Hubbard Model

Balzer, Karsten ; Wolf, F. Alexander ; McCulloch, Ian P. ; Werner, Philipp ; Eckstein, Martin

In: Physical Review X, 2015, vol. 5, no. 3, p. 031039

Symmetry-broken states such as magnetic order and superconductivity are the hallmark of complex properties in solids. An intriguing new direction in condensed-matter physics is to manipulate these properties on the fastest possible time scales using ultrashort laser pulses. Theoretically, however, the collective many-particle dynamics that is responsible for the formation and melting of...

Université de Fribourg

Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

Bachler, Gerald ; Losert, Sabrina ; Umehara, Yuki ; Goetz, Natalie von ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Hungerbuehler, Konrad

In: Particle and Fibre Toxicology, 2015, vol. 12, no. 1, p. 18

The lung epithelial tissue barrier represents the main portal for entry of inhaled nanoparticles (NPs) into the systemic circulation. Thus great efforts are currently being made to determine adverse health effects associated with inhalation of NPs. However, to date very little is known about factors that determine the pulmonary translocation of NPs and their subsequent distribution to secondary...

Université de Fribourg

Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering

Balog, Sandor ; Rodriguez-Lorenzo, Laura ; Monnier, Christophe A. ; Obiols-Rabas, Marc ; Rothen-Rutishauser, Barbara ; Schurtenberger, Peter ; Petri-Fink, Alke

In: Nanoscale, 2015, p. -

Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices – such as biological media and physiological fluids – light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the...

Université de Fribourg

Dynamic depolarized light scattering of small round plasmonic nanoparticles: When imperfection is only perfect

Balog, Sandor ; Rodriguez-Lorenzo, Laura ; Monnier, Christophe A. ; Michen, Benjamin ; Obiols-Rabasa, Marc ; Casal-Dujat, Lucia ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke ; Schurtenberger, Peter

In: The Journal of Physical Chemistry C, 2014, vol. 118, no. 31, p. 17968–17974

Although small round gold nanoparticles (Au NPs) possess only a small degree of shape anisotropy, they support localized surface plasmon resonances and exhibit intrinsic optical anisotropy. These inherent features promote depolarized light scattering, whose temporal fluctuations carry information about rotational Brownian dynamics, and thus can be used to describe the size distribution of...