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Université de Fribourg

TORC1 specifically inhibits microautophagy through ESCRT-0

Hatakeyama, Riko ; Virgilio, Claudio De

In: Current Genetics, 2019, vol. 65, no. 5, p. 1243–1249

Nutrient starvation induces the degradation of specific plasma membrane proteins through the multivesicular body (MVB) sorting pathway and of vacuolar membrane proteins through microautophagy. Both of these processes require the gateway protein Vps27, which recognizes ubiquitinated cargo proteins at phosphatidylinositol 3-phosphate-rich membranes as part of a heterodimeric complex coined...

Université de Fribourg

A spatially and functionally distinct pool of TORC1 defines signaling endosomes in yeast

Hatakeyama, Riko ; De Virgilio, Claudio

In: Autophagy, 2019, vol. 15, no. 5, p. 915–916

The evolutionarily conserved target of rapamycin complex 1 (TORC1) regulates cell growth in a homeostatic manner by tuning anabolic and catabolic processes in response to nutritional and hormonal cues. Interestingly, rather than being localized at the plasma membrane as perhaps expected for an integrator of extracellular signals, TORC1 mainly localizes at vacuolar (in yeast) and lysosomal (in...

Université de Fribourg

Spatially distinct pools of TORC1 balance protein homeostasis

Hatakeyama, Riko ; Péli-Gulli, Marie-Pierre ; Hu, Zehan ; Jaquenoud, Malika ; Osuna, Guillermo Miguel Garcia ; Sardu, Alessandro ; Dengjel, Jörn ; Virgilio, Claudio De

In: Molecular Cell, 2019, vol. 73, no. 2, p. 325-338.e8

The eukaryotic TORC1 kinase is a homeostatic controller of growth that integrates nutritional cues and mediates signals primarily from the surface of lysosomes or vacuoles. Amino acids activate TORC1 via the Rag GTPases that combine into structurally conserved multi-protein complexes such as the EGO complex (EGOC) in yeast. Here we show that Ego1, which mediates membrane-anchoring of EGOC via...

Université de Neuchâtel

The functions of RMR proteins in the "Physcomitrella patens" secretory pathway

Fahr, Noémie ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2017.

Plant vacuoles play a wide range of functions within the cell, from the maintenance of turgor pressure and rigidity, to the storage or degradation of various molecules. Two types of vacuoles with distinct pH can coexist in the same single cell. Acidic vacuoles can be considered as homologues to animal lysosomes while neutral vacuoles are involved in proteins and secondary metabolites storage....

Université de Fribourg

The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability

Wilms, Tobias ; Swinnen, Erwin ; Eskes, Elja ; Dolz-Edo, Laura ; Uwineza, Alice ; Essche, Ruben Van ; Rosseels, Joëlle ; Zabrocki, Piotr ; Cameroni, Elisabetta ; Franssens, Vanessa ; Virgilio, Claudio De ; Smits, Gertien J. ; Winderickx, Joris

In: PLOS Genetics, 2017, vol. 13, no. 6, p. e1006835

The evolutionary conserved TOR complex 1 controls growth in response to the quality and quantity of nutrients such as carbon and amino acids. The protein kinase Sch9 is the main TORC1 effector in yeast. However, only few of its direct targets are known. In this study, we performed a genome-wide screening looking for mutants which require Sch9 function for their survival and growth. In this...

Consortium of Swiss Academic Libraries

Cis-elements of protein transport to the plant vacuoles

Matsuoka, Ken ; Neuhaus, Jean-Marc

In: Journal of Experimental Botany, 1999, vol. 50, no. 331, p. 165-174