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Université de Fribourg

High colonization rate and heterogeneity of ESBL- and carbapenemase-producing enterobacteriaceae isolated from gull feces in Lisbon, Portugal

Aires-de-Sousa, Marta ; Fournier, Claudine ; Lopes, Elizeth ; Lencastre, Hermínia de ; Nordmann, Patrice ; Poirel, Laurent

In: Microorganisms, 2020, vol. 8, no. 10, p. 1487

In order to evaluate whether seagulls living on the Lisbon coastline, Portugal, might be colonized and consequently represent potential spreaders of multidrug-resistant bacteria, a total of 88 gull fecal samples were screened for detection of extended- spectrum β-lactamase (ESBL)- or carbapenemase-producing Enterobacteriaceae for methicillin-resistant Staphylococcus aureus (MRSA) and for...

Université de Fribourg

Epidemiology of carbapenemase-producing Klebsiella pneumoniae in northern Portugal: Predominance of KPC-2 and OXA-48

Lopes, Elizeth ; Saavedra, Maria José ; Costa, Eliana ; Lencastre, Hermíniade ; Poirel, Laurent ; Aires-de-Sousa, Marta

In: Journal of Global Antimicrobial Resistance, 2020, vol. 22, p. 349–353

Objectives: To provide, for the first time, data on the molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae clinical isolates from the northern region of Portugal (Trás-os-Montes and Alto Douro).Methods: A total of 106 carbapenemase-producing K. pneumoniae isolates recovered from clinical samples and rectal swabs between January 2018 and March 2019 were included in this...

Université de Fribourg

Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model

Magalhães, Joana ; Pinheiro, Marina ; Drasler, Barbara ; Septiadi, Dedy ; Petri-Fink, Alke ; Santos, Susana G ; Rothen-Rutishauser, Barbara ; Reis, Salette

In: Nanomedicine, 2020, vol. 15, no. 3, p. 259–271

Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have...

Université de Fribourg

Occurrence of NDM-1-producing Morganella morganii and Proteus mirabilis in a single patient in Portugal: probable in vivo transfer by conjugation

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Goncalves, Maria Luísa ; Costa, Augusto ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2020, vol. 75, no. 4, p. 903–906

To decipher the genetics of acquisition of carbapenemase-encoding genes identified in two carbapenem-resistant Enterobacteriaceae recovered from a single patient in Portugal.Methods: Carbapenemase genes were searched by PCR assays and mating-out assays were performed to further characterize the plasmid support of the carbapenemase genes. Genetic characterization of the plasmid supports was...

Université de Fribourg

Detailed detection of active layer freeze-thaw dynamics using quasi-continuous electrical resistivity tomography (Deception Island, Antarctica)

Farzamian, Mohammad ; Vieira, Gonçalo ; Santos, Fernando A. Monteiro ; Tabar, Borhan Yaghoobi ; Hauck, Christian ; Paz, Maria Catarina ; Bernardo, Ivo ; Ramos, Miguel ; Pablo, Miguel Angel de

In: The Cryosphere, 2020, vol. 14, no. 3, p. 1105–1120

Climate-induced warming of permafrost soils is a global phenomenon, with regional and site-specific variations which are not fully understood. In this context, a 2-D automated electrical resistivity tomography (A-ERT) system was installed for the first time in Antarctica at Deception Island, associated to the existing Crater Lake site of the Circumpolar Active Layer Monitoring – South ...