In: Oncotarget, 2016, vol. 7, no. 18, p. 26628–26652
B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERK-pathway and a pharmacological target of growing clinical relevance, in particular in oncology. To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1...
- Eisenhardt1,2,3, Adrian Sprenger3,5,7, Michael Röring1,2,3,4, Ricarda Herr1,2,3,
Florian Weinberg1,2,3, Martin Köhler1,2,3,4, Sandra Braun1,2,3, Joachim Orth5, Britta
Diedrich3,6, Ulrike Lanner3, Natalja Tscherwinski2,3, Simon Schuster2,3, Nicolas
Dumaz7, Enrico Schmidt2,3, Ralf Baumeister2,3,8,10, Andreas Schlosser2,3,9, Jörn
Dengjel3,6,8,10,11,*, Tilman Brummer1,2,3,10,12,*
1
I
nstitute of Molecular Medicine and Cell Research (IMMZ), Faculty of Medicine, Albert-Ludwigs-University (ALU), Freiburg,
Germany
2
Institute of Biology III, Faculty of Biology, ALU, Freiburg, Germany
3
Centre for Biological Systems Analysis (ZBSA), Freiburg, Germany
4
Spemann Graduate School of Biology and Medicine (SGBM), ALU, Freiburg, Germany
5
Institute for Experimental and Clinical Pharmacology and Toxicology, ALU, Freiburg, Germany
6
Department of Dermatology, University Medical Centre, ALU, Freiburg, Germany
7
INSERM U976 and Université Paris Diderot, Sorbonne Paris Cité, Paris, France
8
Freiburg Institute for Advanced Studies (FRIAS), ALU, Freiburg, Germany
9
Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany
10
Centre for Biological Signalling Studies BIOSS, ALU, Freiburg, Germany
11
Department of Biology, University of Fribourg, Fribourg, Switzerland
12
German Cancer Consortium (DKTK), Freiburg, Germany
*
These authors have contributed equally to this work
Correspondence to: Tilman Brummer, email: tilman.brummer@zbsa.de
Jörn Dengjel, email: joern.dengjel@unifr.ch
Keywords: BRAF, proteomics, phosphorylation, sorafenib, protein-protein interaction
Received: August 18, 2015 Accepted: March 07, 2016 Published: March 28, 2016
ABSTRACT
B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERKpathway and a pharmacological target of growing clinical relevance, in particular in
oncology....
- Galabova-Kovacs G, Kolbus A, Matzen D, Meissl K,
Piazzolla D, Rubiolo C, Steinitz K and Baccarini M....
|