Université de Fribourg

Expression of a microbial serine proteinase inhibitor gene enhances the tobacco defense against oomycete pathogens

Silva, Yussuan ; Portieles, Roxana ; Pujol, Merardo ; Terauchi, Ryohei ; Matsumura, Hideo ; Serrano, Mario ; Borrás-Hidalgo, Orlando

In: Physiological and Molecular Plant Pathology, 2013, vol. 84, p. 99–106

In order to identify Nicotiana megalosiphon genes for novel inhibitors of microbial serine proteinase involved in resistance to the oomycete Phytophthora parasitica var. nicotianae, SuperSAGE technology combined with next-generation sequencing were used to generate libraries in order to identify transcripts that are differentially up-regulated. We identified a N. megalosiphon inhibitor of...

Université de Fribourg

Over-expression of a protein kinase gene enhances the defense of tobacco against Rhizoctonia solani

Chacón, Osmany ; González, Marleny ; López, Yunior ; Portieles, Roxana ; Pujol, Merardo ; González, Ernesto ; Schoonbeek, Henk-Jan ; Métraux, Jean-Pierre ; Borrás-Hidalgo, Orlando

In: Gene, 2010, vol. 45, no. 2, p. 54-62

To identify Nicotiana tabacum genes involved in resistance and susceptibility to Rhizoctonia solani, suppression subtractive hybridization was used to generate a cDNA library from transcripts that are differentially expressed during a compatible and incompatible interaction. This allowed the isolation of a protein kinase cDNA that was down-regulated during a compatible and up-regulated during an...

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2 Portieles, Roxana