Université de Fribourg

Map kinase phosphatase1 and protein tyrosine phosphatase1 are repressors of salicylic acid synthesis and snc1-mediated responses in Arabidopsis

Bartels, Sebastian ; Anderson, Jeffrey C. ; Besteiro González, Marina A. ; Carreri, Alessandro ; Hirt, Heribert ; Buchala, Antony J. ; Métraux, Jean-Pierre ; Peck, Scott C. ; Ulm, Roman

In: The Plant Cell, 2009, vol. 21, p. 2884-2897

Mitogen-activated protein (MAP) kinase phosphatases are important negative regulators of the levels and kinetics of MAP kinase activation that modulate cellular responses. The dual-specificity phosphatase MAP KINASE PHOSPHATASE1 (MKP1) was previously shown to regulate MAP KINASE6 (MPK6) activation levels and abiotic stress responses in Arabidopsis thaliana. Here, we report that the mkp1 null...

Université de Fribourg

Production of reactive oxygen species and wound-induced resistance in Arabidopsis thaliana against Botrytis cinerea are preceded and depend on a burst of calcium

Beneloujaephajri, Emna ; Costa, Alex ; L’Haridon, Floriane ; Métraux, Jean-Pierre ; Binda, Matteo

In: BMC Plant Biology, 2013, vol. 13, no. 1, p. 160

Background: Wounded leaves of Arabidopsis thaliana produce reactive oxygen species (ROS) within minutes after wounding and become resistant to the pathogenic fungus Botrytis cinerea at a local level. This fast response of the plants to the wound is called wound-induced resistance (WIR). However the molecular mechanisms of this response and the signal cascade between the wound and ROS production...

Université de Fribourg

Perception of soft mechanical stress in Arabidopsis leaves activates disease resistance

Benikhlef, Lehcen ; L’Haridon, Floriane ; Abou-Mansour, Eliane ; Serrano, Mario ; Binda, Matteo ; Costa, Alex ; Lehmann, Silke ; Métraux, Jean-Pierre

In: BMC Plant Biology, 2013, vol. 13, no. 1, p. 133

Background: in a previous study we have shown that wounding of Arabidopsis thaliana leaves induces a strong and transient immunity to Botrytis cinerea, the causal agent of grey mould. Reactive oxygen species (ROS) are formed within minutes after wounding and are required for wound–induced resistance to B. cinerea.Results: in this study, we have further explored ROS and resistance to B. cinerea...

Université de Fribourg

A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea

Bessire, Michael ; Chassot, Céline ; Jacquat, Anne-Claude ; Humphry, Matt ; Borel, Sandra ; MacDonald-Comber Petétot, Jean ; Métraux, Jean-Pierre ; Nawrath, Christiane

In: The EMBO Journal, 2007, vol. 26, no. 8, p. 2158–2168

The plant cuticle composed of cutin, a lipid-derived polyester, and cuticular waxes covers the aerial portions of plants and constitutes a hydrophobic extracellular matrix layer that protects plants against environmental stresses. The botrytis-resistant 1 (bre1) mutant of Arabidopsis reveals that a permeable cuticle does not facilitate the entry of fungal pathogens in...

Université de Fribourg

A member of the PLEIOTROPIC DRUG RESISTANCE family of ATP binding cassette transporters is required for the formation of a functional cuticle in Arabidopsis

Bessire, Michael ; Borel, Sandra ; Fabre, Guillaume ; Carraça, Luis ; Efremova, Nadia ; Yephremov, Alexander ; Cao, Yan ; Jetter, Reinhard ; Jacquat, Anne-Claude ; Métraux, Jean-Pierre ; Nawrath, Christiane

In: Plant Cell, 2011, vol. 23, no. 5, p. 1958-1970

Although the multilayered structure of the plant cuticle was discovered many years ago, the molecular basis of its formation and the functional relevance of the layers are not understood. Here, we present the permeable cuticle1 (pec1) mutant of Arabidopsis thaliana, which displays features associated with a highly permeable cuticle in several organs. In...

Université de Fribourg

The cuticle mutant eca2 modifies plant defense responses to biotrophic and necrotrophic pathogens and herbivory insects

Blanc, Catherine ; Coluccia, Fania ; L?Haridon, Floriane ; Torres, Martha ; Ortiz-Berrocal, Marlene ; Stahl, Elia ; Reymond, Philippe ; Schreiber, Lukas ; Nawrath, Christiane ; Métraux, Jean-Pierre ; Serrano, Mario

In: Molecular Plant-Microbe Interactions, 2017, vol. 31, no. 3, p. 344–355

We isolated previously several Arabidopsis thaliana mutants with constitutive expression of the early microbe-associated molecular pattern–induced gene ATL2, named eca (expresión constitutiva de ATL2). Here, we further explored the interaction of eca mutants with pest and pathogens. Of all eca mutants, eca2 was more resistant to a fungal pathogen (Botrytis cinerea) and a bacterial pathogen...

Université de Fribourg

The glutaredoxin ATGRXS13 is required to facilitate Botrytis cinerea infection of Arabidopsis thaliana plants

Camera, Sylvain La ; L’Haridon, Floriane ; Astier, Jérémy ; Zander, Mark ; Abou-Mansour, Eliane ; Page, Gonzague ; Thurow, Corinna ; Wendehenne, David ; Gatz, Christiane ; Métraux, Jean-Pierre ; Lamotte, Olivier

In: The Plant Journal, 2011, vol. 68, no. 3, p. 507–519

Botrytis cinerea is a major pre- and post-harvest necrotrophic pathogen with a broad host range that causes substantial crop losses. The plant hormone jasmonic acid (JA) is involved in the basal resistance against this fungus. Despite basal resistance, virulent strains of B. cinerea can cause disease on Arabidopsis thaliana and virulent pathogens can interfere with the metabolism of the host in a...

Université de Fribourg

Gene-specific Involvement of ß-oxidation in wound-activated responses in Arabidopsis

Castillo, M. Cruz ; Martínez, Cristina ; Buchala, Antony ; Métraux, Jean-Pierre ; León, José

In: Plant Physiology, 2004, vol. 135, p. 95-104

The coordinated induced expression of ß-oxidation genes is essential to provide the energy supply for germination and postgerminative development. However, very little is known about other functions of ß-oxidation in nonreserve organs. We have identified a gene-specific pattern of induced ß-oxidation gene expression in wounded leaves of Arabidopsis. Mechanical damage triggered the local and...

Université de Fribourg

Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana

Catinot, Jérémy ; Buchala, Antony ; Abou-Mansour, Eliane ; Métraux, Jean-Pierre

In: Febs Letters, 2008, vol. 582, no. 4, p. 473-478

Salicylic acid (SA) is an important signal involved in the activation of defence responses against abiotic and biotic stress. In tobacco, benzoic acid or glucosyl benzoate were proposed to be precursors of SA. This is in sharp contrast with studies in Arabidopsis thaliana, where SA derives from isochorismate. We have determined the importance of isochorismate for SA biosynthesis in Nicotiana...

Université de Fribourg

Over-expression of a protein kinase gene enhances the defense of tobacco against Rhizoctonia solani

Chacón, Osmany ; González, Marleny ; López, Yunior ; Portieles, Roxana ; Pujol, Merardo ; González, Ernesto ; Schoonbeek, Henk-Jan ; Métraux, Jean-Pierre ; Borrás-Hidalgo, Orlando

In: Gene, 2010, vol. 45, no. 2, p. 54-62

To identify Nicotiana tabacum genes involved in resistance and susceptibility to Rhizoctonia solani, suppression subtractive hybridization was used to generate a cDNA library from transcripts that are differentially expressed during a compatible and incompatible interaction. This allowed the isolation of a protein kinase cDNA that was down-regulated during a compatible and up-regulated during an...