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    Université de Neuchâtel

    Stage 0 sporulation gene A as a molecular marker to study diversity of endospore-forming Firmicutes

    Wunderlin, Tina ; Junier, Thomas ; Roussel-Delif, Ludovic ; Jeanneret, Nicole ; Junier, Pilar

    In: Environmental Microbiology Reports, 2013, vol. 5, no. 6, p. 911-924

    In this study, we developed and validated a cultureindependent method for diversity surveys to specifically detect endospore-forming Firmicutes. The global transcription regulator of sporulation (spo0A) was identified as a gene marker for endosporeforming Firmicutes. To enable phylogenetic classification, we designed a set of primers amplifying a 602 bp fragment of spo0A that we...

    Université de Neuchâtel

    Quantification of Endospore-Forming Firmicutes by Quantitative PCR with the Functional Gene spo0A

    Bueche, Matthieu ; Wunderlin, Tina ; Roussel-Delif, Ludovic ; Junier, Thomas ; Sauvain, Loïc ; Jeanneret, Nicole ; Junier, Pilar

    In: Applied and Environmental Microbiology, 2013, vol. 79, no. 17, p. 5302-5312

    Bacterial endospores are highly specialized cellular forms that allow endospore-forming Firmicutes (EFF) to tolerate harsh environmental conditions. EFF are considered ubiquitous in natural environments, in particular, those subjected to stress conditions. In addition to natural habitats, EFF are often the cause of contamination problems in anthropogenic environments, such as industrial...