Public access from Aug 17, 2020
Università della Svizzera italiana

Imaging cell interaction in tracheal mucosa during influenza virus infection using two-photon intravital microscopy

Palomino-Segura, Miguel ; Virgilio, Tommaso ; Morone, Diego ; Pizzagalli, Diego U. ; Gonzalez, Santiago F.

In: Journal of visualized experiments, 2018, vol. 138, p. e58355

The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics of the immune response to infection. Two-photon intravital microscopy (2P-IVM) allows the observation of cell interactions in deep tissue in living animals, while minimizing the photobleaching generated during image acquisition. To date, different models for 2P-IVM of lymphoid and...