Université de Neuchâtel

Variability and Action Mechanism of a Family of Anticomplement Proteins in Ixodes ricinus

Couvreur, Bernard ; Beaufays, Jérôme ; Charon, Cédric ; Lahaye, Kathia ; Gensale, François ; Denis, Valérie ; Charloteaux, Benoît ; Decrem, Yves ; Prévôt, Pierre-Paul ; Brossard, Michel ; Vanhamme, Luc ; Godfroid, Edmond

In: PLoS One, 2008, vol. 3, no. 1, p. e1400 1-24

Background : Ticks are blood feeding arachnids that characteristically take a long blood meal. They must therefore counteract host defence mechanisms such as hemostasis, inflammation and the immune response. This is achieved by expressing batteries of salivary proteins coded by multigene families. Methodology/Principal Findings : We report the in-depth analysis of...

Université de Neuchâtel

The impact of gene knock-down and vaccination against salivary metalloproteases on blood feeding and egg laying by Ixodes ricinus

Decrem, Yves ; Mariller, Marcel ; Lahaye, Kathia ; Blasioli, Virginie ; Beaufays, Jérôme ; Boudjeltia, Karim Zouaoui ; Vanhaeverbeek, Michel ; Cérutti, Martine ; Brossard, Michel ; Vanhamme, Luc ; Godfroid, Edmond

In: International Journal for Parasitology, 2008, vol. 38, no. 5, p. 549-560

Two cDNAs coding homologous putative metalloproteases (Metis 1 and Metis 2, expected molecular weights of 55.6 and 56.0 kDa, respectively) were identified from the hard tick Ixodes ricinus. The expression of Metis genes was induced in salivary glands during tick blood meal. RNA interference was used to assess the role of both Metis 1 and Metis 2 in tick feeding. It was found that salivary...