Université de Neuchâtel

Characterization of a Novel Salivary Immunosuppressive Protein from Ixodes ricinus Ticks

Leboulle, Gérard ; Crippa, Mara ; Decrem, Yves ; Mejri, Naceur ; Brossard, Michel ; Bollen, Alex ; Godfroid, Edmond

In: Journal of Biological Chemistry, 2002, vol. 277, no. 12, p. 10083-10089

In tick salivary glands, several genes are induced during the feeding process, leading to the expression of new proteins. These proteins are typically secreted in tick saliva and are potentially involved in the modulation of the host immune and hemostatic responses. In a previous study, the construction and the analysis of a subtractive library led to the identification of Ixodes...

Université de Neuchâtel

A family of putative metalloproteases in the salivary glands of the tick Ixodes ricinus

Decrem, Yves ; Beaufays, Jérôme ; Blasioli, Virginie ; Brossard, Michel ; Vanhamme, Luc ; Godfroid, Edmond

In: FEBS Journal, 2008, vol. 275, no. 7, p. 1485-1499

Ticks are obligate blood-feeding arachnids. During their long-lasting blood meal, they have to counteract the protective barriers and defense mechanisms of their host. These include tissue integrity, pain, hemostasis, and the inflammatory and immune reactions. Here, we describe a multigene family coding for five putative salivary metalloproteases induced during the blood meal of Ixodes...

Université de Neuchâtel

Variability and Action Mechanism of a Family of Anticomplement Proteins in Ixodes ricinus

Couvreur, Bernard ; Beaufays, Jérôme ; Charon, Cédric ; Lahaye, Kathia ; Gensale, François ; Denis, Valérie ; Charloteaux, Benoît ; Decrem, Yves ; Prévôt, Pierre-Paul ; Brossard, Michel ; Vanhamme, Luc ; Godfroid, Edmond

In: PLoS One, 2008, vol. 3, no. 1, p. e1400 1-24

Background : Ticks are blood feeding arachnids that characteristically take a long blood meal. They must therefore counteract host defence mechanisms such as hemostasis, inflammation and the immune response. This is achieved by expressing batteries of salivary proteins coded by multigene families. Methodology/Principal Findings : We report the in-depth analysis of...

Université de Neuchâtel

Ixodes ricinus Tick Lipocalins: Identification, Cloning, Phylogenetic Analysis and Biochemical Characterization

Beaufays, Jérôme ; Adam, Benoît ; Decrem, Yves ; Prévôt, Pierre-Paul ; Santini, Sébastien ; Brasseur, Robert ; Brossard, Michel ; Lins, Laurence ; Vanhamme, Luc ; Godfroid, Edmond

In: PLoS One, 2008, vol. 3, no. 12, p. e3941 1-11

Background : During their blood meal, ticks secrete a wide variety of proteins that interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of the inflammatory response. Methodology/Principal Findings : Screening a cDNA library in association with RT-PCR and RACE methodologies allowed us to identify 14...

Université de Neuchâtel

Ir-LBP, an Ixodes ricinus Tick Salivary LTB4-Binding Lipocalin, Interferes with Host Neutrophil Function

Beaufays, Jérôme ; Adam, Benoît ; Menten-Dedoyart, Catherine ; Fievez, Laurence ; Grosjean, Amélie ; Decrem, Yves ; Prévôt, Pierre-Paul ; Santini, Sébastien ; Brasseur, Robert ; Brossard, Michel ; Vanhaeverbeek, Michel ; Bureau, Fabrice ; Heinen, Ernst ; Lins, Laurence ; Vanhamme, Luc ; Godfroid, Edmond

In: PLoS One, 2008, vol. 3, no. 12, p. e3987 1-13

Background : During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of the inflammatory response. Methodology/Principal Findings : We previously identified 14 new lipocalin genes in the tick Ixodes ricinus. One of them...

Université de Neuchâtel

The impact of gene knock-down and vaccination against salivary metalloproteases on blood feeding and egg laying by Ixodes ricinus

Decrem, Yves ; Mariller, Marcel ; Lahaye, Kathia ; Blasioli, Virginie ; Beaufays, Jérôme ; Boudjeltia, Karim Zouaoui ; Vanhaeverbeek, Michel ; Cérutti, Martine ; Brossard, Michel ; Vanhamme, Luc ; Godfroid, Edmond

In: International Journal for Parasitology, 2008, vol. 38, no. 5, p. 549-560

Two cDNAs coding homologous putative metalloproteases (Metis 1 and Metis 2, expected molecular weights of 55.6 and 56.0 kDa, respectively) were identified from the hard tick Ixodes ricinus. The expression of Metis genes was induced in salivary glands during tick blood meal. RNA interference was used to assess the role of both Metis 1 and Metis 2 in tick feeding. It was found that salivary...

Université de Neuchâtel

Anti-hemostatic Effects of a Serpin from the Saliva of the Tick Ixodes ricinus

Prevot, Pierre-Paul ; Adam, Benoit ; Zouaoui Boudjeltia, Karim ; Brossard, Michel ; Lins, Laurence ; Cauchie, Philippe ; Brasseur, Robert ; Vanhaeverbeek, Michel ; Vanhamme, Luc ; Godfroid, Edmond

In: Journal of Biological Chemistry, 2006, vol. 281, no. 36, p. 26361-26369

Serpins (serine protease inhibitors) are a large family of structurally related proteins found in a wide variety of organisms, including hematophagous arthropods. Protein analyses revealed that Iris, previously described as an immunomodulator secreted in the tick saliva, is related to the leukocyte elastase inhibitor and possesses serpin motifs, including the reactive center...

Université de Neuchâtel

Isolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding

Leboulle, Gérard ; Rochez, Candice ; Louahed, Jamila ; Rutti, Bernard ; Brossard, Michel ; Bollen, Alex ; Godfroid, Edmond

In: American Journal of Tropical Medicine and Hygiene, 2002, vol. 66, no. 3, p. 225-233

In tick salivary glands, genes induced during blood feeding result in the expression of new proteins secreted into tick saliva. These proteins are potentially involved in modulation of vertebrate host immune and hemostatic responses. In this study, subtractive and full-length cDNA libraries were constructed by use of mRNA extracted from salivary glands of unfed and 5-day engorged Ixodes...