Université de Fribourg

Deregulation of MADS-box transcription factor genes in a mutant defective in the WUSCHEL-LIKE HOMEOBOX gene EVERGREEN of Petunia hybrida

Schorderet, Martine ; Muni, Rajasekhara Reddy Duvvuru ; Fiebig, A. ; Reinhardt, Didier

In: Plant Signaling & Behavior, 2018, vol. 13, no. 6, p. e1471299

Angiosperm inflorescences develop in two fundamentally different ways. In monopodial plants, for example in Arabidopsis thaliana, the flowers are initiated as lateral appendages of a central indeterminate inflorescence meristem. In sympodial plants, flowers arise by terminal differentiation of the inflorescence meristem, while further inflorescence development proceeds from new sympodial...

Université de Fribourg

An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution

Robinson, Sarah ; Huflejt, Michal ; Barbier de Reuille, Pierre ; Braybrook, Siobhan A. ; Schorderet, Martine ; Reinhardt, Didier ; Kuhlemeier, Cris

In: The Plant Cell, 2017, vol. 29, no. 12, p. 2959–2973

How complex developmental-genetic networks are translated into organs with specific 3D shapes remains an open question. This question is particularly challenging because the elaboration of specific shapes is in essence a question of mechanics. In plants, this means how the genetic circuitry affects the cell wall. The mechanical properties of the wall and their spatial variation are the key...

Université de Fribourg

A petunia GRAS transcription factor controls symbiotic gene expression and fungal morphogenesis in arbuscular mycorrhiza

Rich, Mélanie, K ; Schorderet, Martine ; Bapaume, Laure ; Falquet, Laurent ; Morel, Patrice ; Vandenbussche, Michiel ; Reinhardt, Didier

In: Plant Physiology, 2015, p. -

Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves a complex symbiotic interface over which nutrients are exchanged between the plant host and the AM fungus. Dozens of genes in the host are required for the establishment and functioning of the interaction, among them nutrient transporters that mediate the uptake of mineral nutrients delivered by the fungal arbuscules. We have isolated...

Université de Fribourg

The role of the cell wall compartment in mutualistic symbioses of plants

Rich, Mélanie K. ; Schorderet, Martine ; Reinhardt, Didier

In: Plant-Microbe Interaction, 2014, vol. 5, p. 238

Plants engage in mutualistic interactions with microbes that improve their mineral nutrient supply. The most wide-spread symbiotic association is arbuscular mycorrhiza (AM), in which fungi of the order Glomeromycota invade roots and colonize the cellular lumen of cortical cells. The establishment of this interaction requires a dedicated molecular-genetic program and a cellular machinery of the...

Université de Fribourg

Elastic domains regulate growth and organogenesis in the plant shoot apical meristem

Kierzkowski, Daniel ; Nakayama, Naomi ; Routier-Kierzkowska, Anne-Lise ; Weber, Alain ; Bayer, Emmanuelle ; Schorderet, Martine ; Reinhardt, Didier ; Kuhlemeier, Cris ; Smith, Richard S.

In: Science, 2012, vol. 335, p. 1096

Although genetic control of morphogenesis is well established, elaboration of complex shapes requires changes in the mechanical properties of cells. In plants, the first visible sign of leaf formation is a bulge on the flank of the shoot apical meristem. Bulging results from local relaxation of cell walls, which causes them to yield to internal hydrostatic pressure. By manipulation of tissue...

Université de Fribourg

The PAM1 gene of petunia, required for intracellular accommodation and morphogenesis of arbuscular mycorrhizal fungi, encodes a homologue of VAPYRIN

Feddermann, Nadja ; Duvvuru Muni, Rajasekhara Reddy ; Zeier, Tatyana ; Stuurman, Jeroen ; Ercolin, Flavia ; Schorderet, Martine ; Reinhardt, Didier

In: The Plant Journal, 2011, vol. 64, no. 3, p. 470–481

Most terrestrial plants engage into arbuscular mycorrhizal (AM) symbiosis with fungi of the phylum Glomeromycota. The initial recognition of the fungal symbiont results in the activation of a symbiosis signalling pathway that is shared with the root nodule symbiosis (common SYM pathway). The subsequent intracellular accommodation of the fungus, and the elaboration of its characteristic feeding...

Université de Fribourg

Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase

Streb, Sebastian ; Delatte, Thierry ; Umhang, Martin ; Eicke, Simona ; Schorderet, Martine ; Reinhardt, Didier ; Zeeman, Samuel C.

In: The Plant Cell, 2009, vol. 20, p. 3448-3466

Several studies have suggested that debranching enzymes (DBEs) are involved in the biosynthesis of amylopectin, the major constituent of starch granules. Our systematic analysis of all DBE mutants of Arabidopsis thaliana demonstrates that when any DBE activity remains, starch granules are still synthesized, albeit with altered amylopectin structure. Quadruple mutants lacking all four DBE proteins...

Université de Fribourg

A petunia mutant affected in intracellular accommodation and morphogenesis of arbuscular mycorrhizal fungi

Reddy, Sekhara ; Schorderet, Martine ; Feller, Urs ; Reinhardt, Didier

In: The Plant Journal, 2007, vol. 51, no. 5, p. 739-750

The regulation of the arbuscular mycorrhizal (AM) symbiosis is largely under the control of a genetic programme of the plant host. This programme includes a common symbiosis signalling pathway that is shared with the root nodule symbiosis. Whereas this common pathway has been investigated in detail, little is known about the mycorrhiza-specific regulatory steps upstream and downstream of the...

Université de Fribourg

Induction of differentiation in the shoot apical meristem by transient overexpression of a retinoblastoma-related protein

Wyrzykowska, Joanna ; Schorderet, Martine ; Pien, Stéphane ; Gruissem, Wilhelm ; Fleming, Andrew J.

In: Plant Physiology, 2006, vol. 141, no. 4, p. 1338-1348

The shoot apical meristem contains cells that undergo continual growth and division to generate the building blocks for the aerial portion of the plant. As cells leave the meristem, they undergo differentiation to form specific cell types. Most notably, heterotrophic cells of the meristem rapidly gain autotrophic capability by synthesis and assembly of components of the chloroplast. At the same...

Université de Fribourg

Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions

Sieber, Patrick ; Schorderet, Martine ; Ryser, Ulrich ; Buchala, Antony ; Kolattukudy, Pappachan ; Métraux, Jean-Pierre ; Nawrath, Christiane

In: Plant Cell, 2000, vol. 12, p. 721

A major structural component of the cuticle of plants is cutin. Analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. Therefore, transgenic Arabidopsis plants were generated that express and secrete a cutinase from Fusarium solani f sp pisi. Arabidopsis plants expressing the cutinase in the extracellular...