Université de Fribourg

Disease resistance of Arabidopsis to Phytophthora brassicae is established by the sequential action of indole glucosinolates and camalexin

Schlaeppi, Klaus ; Abou-Mansour, Eliane ; Buchala, Antony J. ; Mauch, Felix

In: The Plant Journal, 2010, vol. 62, no. 5, p. 840 - 851

We have analysed the role of tryptophan-derived secondary metabolites in disease resistance of Arabidopsis to the oomycete pathogen Phytophthora brassicae. Transcript analysis revealed that genes encoding enzymes involved in tryptophan, camalexin and indole glucosinolate (iGS) biosynthesis are coordinately induced in response to P. brassicae. However, a deficiency in either camalexin or iGS...

Université de Fribourg

Subcellular immunocytochemical analysis detects the highest concentrations of glutathione in mitochondria and not in plastids

Zechmann, B. ; Mauch, Felix ; Sticher, Liliane ; Müller, M.

In: Journal of Experimental Botany, 2008, vol. 59, no. 14, p. 4017-4027

The tripeptide glutathione is a major antioxidant and redox buffer with multiple roles in plant metabolism. Glutathione biosynthesis is restricted to the cytosol and the plastids and the product is distributed to the various organelles by unknown mechanisms. In the present study immunogold cytochemistry based on anti-glutathione antisera and transmission electron microscopy was used to...

Université de Fribourg

Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism

Glombitza, Sabine ; Dubuis, Pierre-henri ; Thulke, Oliver ; Welzl, Gerhard ; Bovet, Lucien ; Götz, Michael ; Affenzeller, Matthias ; Geist, Birgit ; Hehn, Alain ; Asnaghi, Carole ; Ernst, Dieter ; Seidlitz, Harald ; Gundlach, Heidrun ; Mayer, Klaus ; Martinoia, Enrico ; Werck-reichhart, Daniele ; Mauch, Felix ; Schäffner, Anton

In: Plant Molecular Biology, 2005, vol. 54, p. 817-835

Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively in secondary metabolism and defense reactions, a DNA array (MetArray) harboring gene-specific probes was established. It comprised complete sets...