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Université de Fribourg

Contribution of hydrogen cyanide to the antagonistic activity of Pseudomonas strains against Phytophthora infestans

Anand, Abhishek ; Chinchilla, Delphine ; Tan, Christopher ; Mène-Saffrané, Laurent ; L’Haridon, Floriane ; Weisskopf, Laure

In: Microorganisms, 2020, vol. 8, no. 8, p. 1144

Plants face many biotic and abiotic challenges in nature; one of them is attack by disease-causing microbes. Phytophthora infestans, the causal agent of late blight is one of the most prominent pathogens of the potato responsible for multi-billion-dollar losses every year. We have previously reported that potato-associated Pseudomonas strains inhibited P. infestans at various developmental...

Université de Fribourg

Identification of a species-specific aminotransferase in Pediococcus acidilactici capable of forming α-aminobutyrate

Wenger, Alexander ; Schmidt, Remo S. ; Portmann, Reto ; Roetschi, Alexandra ; Eugster, Elisabeth ; Weisskopf, Laure ; Irmler, Stefan

In: AMB Express, 2020, vol. 10, no. 1, p. 100

During cheese ripening, the bacterial strain Pediococcus acidilactici FAM18098 produces the non-proteinogenic amino acid, α-aminobutyrate (AABA). The metabolic processes that lead to the biosynthesis of this compound are unknown. In this study, 10 P. acidilactici, including FAM18098 and nine Pediococcus pentosaceus strains, were screened for their ability to produce AABA. All P. acidilactici...

Université de Fribourg

S-methyl methanethiosulfonate: promising late blight inhibitor or broad range toxin?

Joller, Charlotte ; Vrieze, Mout De ; Moradi, Aboubakr ; Fournier, Claudine ; Chinchilla, Delphine ; L’Haridon, Floriane ; Bruisson, Sebastien ; Weisskopf, Laure

In: Pathogens, 2020, vol. 9, no. 6, p. 496

(1) Background: S-methyl methanethiosulfonate (MMTS), a sulfur containing volatile organic compound produced by plants and bacterial species, has recently been described to be an efficient anti-oomycete agent with promising perspectives for the control of the devastating potato late blight disease caused by Phytophthora infestans. However, earlier work raised questions regarding the putative...

Université de Fribourg

Linking comparative genomics of nine potato-associated pseudomonas isolates with their differing biocontrol potential against late blight

Vrieze, Mout De ; Varadarajan, Adithi R. ; Schneeberger, Kerstin ; Bailly, Aurélien ; Rohr, Rudolf P. ; Ahrens, Christian H. ; Weisskopf, Laure

In: Frontiers in Microbiology, 2020, vol. 11, p. 857

For plants, the advantages of associating with beneficial bacteria include plant growth promotion, reduction of abiotic and biotic stresses and enhanced protection against various pests and diseases. Beneficial bacteria rightly equipped for successful plant colonization and showing antagonistic activity toward plant pathogens seem to be actively recruited by plants. To gain more insights into...

Université de Fribourg

Genome insights of the plant-growth promoting bacterium Cronobacter muytjensii JZ38 with volatile-mediated antagonistic activity against Phytophthora infestans

Eida, Abdul Aziz ; Bougouffa, Salim ; L’Haridon, Floriane ; Alam, Intikhab ; Weisskopf, Laure ; Bajic, Vladimir B. ; Saad, Maged M. ; Hirt, Heribert

In: Frontiers in Microbiology, 2020, vol. 11, p. 369

Salinity stress is a major challenge to agricultural productivity and global food security in light of a dramatic increase of human population and climate change. Plant growth promoting bacteria can be used as an additional solution to traditional crop breeding and genetic engineering. In the present work, the induction of plant salt tolerance by the desert plant endophyte Cronobacter sp. ...

Université de Fribourg

Endophytes and epiphytes from the grapevine leaf microbiome as potential biocontrol agents against phytopathogens

Bruisson, Sébastien ; Zufferey, Mónica ; L’Haridon, Floriane ; Trutmann, Eva ; Anand, Abhishek ; Dutartre, Agnès ; Vrieze, Mout De ; Weisskopf, Laure

In: Frontiers in Microbiology, 2019, vol. 10, p. -

Plants harbor diverse microbial communities that colonize both below-ground and above-ground organs. Some bacterial members of these rhizosphere and phyllosphere microbial communities have been shown to contribute to plant defenses against pathogens. In this study, we characterize the pathogen-inhibiting potential of 78 bacterial isolates retrieved from endophytic and epiphytic communities...

Université de Fribourg

Airborne medicine: bacterial volatiles and their influence on plant health

Garbeva, Paolina ; Weisskopf, Laure

In: New Phytologist, 2019, p. -

Like most other eukaryotes, plants do not live alone but in close association with a diverse microflora. These plant‐associated microbes contribute to plant health in many different ways, ranging from modulation of hormonal pathways to direct antibiosis of plant pathogens. Over the last 15 yr, the importance of volatile organic compounds as mediators of mutualistic interactions between...

Université de Fribourg

Biocontrol activity of three Pseudomonas in a newly assembled collection of Phytophthora infestans isolates

De Vrieze, Mout ; Gloor, Ramona ; Massana Codina, Josep ; Torriani, Stefano ; Gindro, Katia ; L?Haridon, Floriane ; Bailly, Aurélien ; Weisskopf, Laure

In: Phytopathology, 2019, vol. 109, no. 9, p. 1555–1565

Late blight caused by the oomycete Phytophthora infestans constitutes the greatest threat to potato production worldwide. Considering the increasing concerns regarding the emergence of novel fungicide-resistant genotypes and the general demand for reducing inputs of synthetic and copper-based fungicides, the need for alternative control methods is acute. Several bacterial antagonists have...

Université de Fribourg

Microbial life in the grapevine: what can we expect from the leaf microbiome?

Vionnet, Léo ; Vrieze, Mout De ; Dutartre, Agnès ; Gfeller, Aurélie ; Lüthi, Angelika ; L’Haridon, Floriane ; Weisskopf, Laure

In: OENO One, 2018, vol. 52, no. 3, p. 219–224

Recent studies have shown that plants harbor complex bacterial communities, the so- called “microbiome”. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which, in analogy to the gut microbiome, are likely to be of essential nature. The aim of this work was to analyze the abundance and diversity of the cultivable...

Université de Fribourg

Combining different potato-associated pseudomonas strains for improved biocontrol of Phytophthora infestans

Vrieze, Mout De ; Germanier, Fanny ; Vuille, Nicolas ; Weisskopf, Laure

In: Frontiers in Microbiology, 2018, vol. 9, p. -

Late blight caused by Phytophthora infestans is considered as the most devastating disease of potato and is a re-emerging problem worldwide. Current late blight control practices rely mostly on synthetic fungicides or copper-based products, but growing awareness of the negative impact of these compounds on the environment has led to the search for alternative control measures. A collection of...