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Université de Fribourg

Phenotypic, biochemical, and genetic analysis of kpc-41, a kpc-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity

Mueller, Linda ; Masseron, Amandine ; Prod’Hom, Guy ; Galperine, Tatiana ; Greub, Gilbert ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 12, p. -

A novel KPC variant, KPC-41, was identified in a Klebsiella pneumoniae clinical isolate from Switzerland. This β-lactamase possessed a 3-amino-acid insertion (Pro- Asn-Lys) located between amino acids 269 and 270 compared to the KPC-3 amino acid sequence. Cloning and expression of the blaKPC-41 gene in Escherichia coli, followed by determination of MIC values and kinetic parameters, showed...

Université de Fribourg

Identification of fosa8, a plasmid-encoded fosfomycin resistance determinant from Escherichia coli, and its origin in Leclercia adecarboxylata

Poirel, Laurent ; Vuillemin, Xavier ; Kieffer, Nicolas ; Mueller, Linda ; Descombes, Marie-Christine ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 11, p. -

A plasmid-located fosfomycin resistance gene, fosA8, was identified from a CTX-M- 15-producing Escherichia coli isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The fosA8 gene was located on a transferable 50-kb...

Université de Fribourg

Evaluation of resazurin-based rapid test to detect colistin resistance in Acinetobacter baumannii isolates

Germ, Julija ; Poirel, Laurent ; Cerar Kisek, Tjasa ; Cvitkovic Spik, Vesna ; Katja Seme ; Mueller Premru, Manica ; Lejko Zupanc, Tatjana ; Nordmann, Patrice ; Pirs, Mateja

In: European Journal of Clinical Microbiology & Infectious Diseases, 2019, vol. 38, no. 11, p. 2159–2162

Acinetobacter baumannii primarily causes colonization, yet it can be an opportunistic pathogen associated with hospital-acquired infections. Many countries report rapid spread of carbapenem-resistant Acinetobacter baumannii (CRAb) which limits treatment options, with colistin frequently being the last line treatment option. The aim of our study was to evaluate a recently developed rapid...

Université de Fribourg

CTX-M-33 Is a CTX-M-15 derivative conferring reduced susceptibility to carbapenems

Poirel, Laurent ; Rosa, c José-Manuel Ortiz de la ; Richard, Anaïs ; Aires-de-Sousa, Marta ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 12, p. -

CTX-M-type extended-spectrum β-lactamases (ESBLs) are widespread among Enterobacterales strains worldwide. The most common variant is CTX-M-15, which hydrolyzes ceftazidime at a high rate but spares carbapenems. Here, we identified CTX-M-33, a point mutation derivative of CTX-M-15 (Asp to Ser substitution at Ambler position 109) that exhibited low carbapenemase activity. β-Lactamase...

Université de Fribourg

ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans

Kieffer, Nicolas ; Guzmán-Puche, Julia ; Poirel, Laurent ; Kang, Hyo Jung ; Jeon, Che Ok ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1568–1571

Objectives: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment.Methods: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme.Results: The enzyme named ZHO-1...

Université de Fribourg

A selective culture medium for screening linezolid-resistant gram-positive bacteria

Nordmann, Patrice ; Rodríguez-Villodres, Angel ; Poirel, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2019, vol. 95, no. 1, p. 1–4

The SuperLinezolid medium was developed for screening resistance to linezolid (LZD) in Gram-positive bacteria (Staphylococcus spp., Enterococcus spp.). It was evaluated using LZD-susceptible (n = 20) and LZD-resistant (n = 17) Gram-positive isolates. The sensitivity was found to be 82% at 24 h (3 out of 17 isolates being missed), and reached 100% at 48 h. At 48 h, a single...

Université de Fribourg

mcr-9, An inducible gene encoding an acquired phosphoethanolamine transferase in Escherichia coli, and its origin

Kieffer, Nicolas ; Royer, b Guilhem ; Decousser, Jean-Winoc ; Bourrel, Anne-Sophie ; Palmieri, Mattia ; Ortiz De La Rosa, Jose-Manuel ; Jacquier, Hervé ; Denamur, Erick ; Nordmann, Patrice ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 9, p. -

The plasmid-located mcr-9 gene, encoding a putative phosphoethanolamine transferase, was identified in a colistin-resistant human fecal Escherichia coli strain belonging to a very rare phylogroup, the D-ST69-O15:H6 clone. This MCR-9 protein shares 33% to 65% identity with the other plasmid-encoded MCR-type enzymes identified (MCR-1 to -8) that have been found as sources of acquired resistance...

Université de Fribourg

Epidemiology of Carbapenemase-Producing Klebsiella pneumoniae in a Hospital, Portugal

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Gonçalves, Maria Luísa ; Pereira, Ana Luísa ; Nordmann, Patrice ; Poirel, Laurent

In: Emerging Infectious Diseases, 2019, vol. 25, no. 9, p. 1632–1638

We aimed to provide updated epidemiologic data on carbapenem-resistant Klebsiella pneumoniae in Portugal by characterizing all isolates (N = 46) recovered during 2013– 2018 in a 123-bed hospital in Lisbon. We identified blaKPC-3 (n = 36), blaOXA-181 (n = 9), and blaGES-5 (n = 8) carbapenemase genes and observed co-occurrence of blaKPC-3 and blaGES-5 in 7 isolates. A single GES-5–producing...

Université de Fribourg

Efficacy of colistin alone and in various combinations for the treatment of experimental osteomyelitis due to carbapenemase-producing Klebsiella pneumoniae

Crémieux, Anne-Claude ; Dinh, Aurélien ; Nordmann, Patrice ; Mouton, William ; Tattevin, Pierre ; Ghout, Idir ; Jayol, Aurelie ; Aimer, Omar ; Gatin, Laure ; Verdier, Marie-Clémence ; Saleh-Mghir, Azzam ; Laurent, Frédéric

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 9, p. 2666–2675

Objectives: In a new experimental model of carbapenemase-producing Klebsiella pneumoniae osteomyelitis we evaluated the efficacy of colistin alone and in various combinations and examined the emergence of colistin-resistant strains and cross- resistance to host defence peptides (HDPs).Methods: KPC-99YC is a clinical strain with intermediate susceptibility to meropenem (MIC = 4 mg/L) and...

Université de Fribourg

Functional characterization of a miniature inverted transposable element at the origin of mcr-5 gene acquisition in Escherichia coli

Kieffer, Nicolas ; Nordmann, Patrice ; Millemann, Yves ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019/63/7/e00559-19, /aac/63/7/AAC.00559-19.atom

Plasmid-mediated colistin resistance of the mobile colistin resistance (MCR) type is a growing concern in Enterobacteriaceae since it has been described worldwide in humans and animals. Here, we identified a series of MCR-producing Escherichia coli isolates corresponding to two different clones (represented by isolates PS1 and PS8b) producing MCR-1 and MCR-5, respectively, obtained from pig...