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Development of a molecular method for enumerating viable Ascaris eggs from feacal sludge

Rabenifara Estoppey, Antsa ; Schmid, Sergio (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2015.

This study is a part of a project that aims to set up a molecular method to evaluate the viability of Ascaris eggs during sludge treatment. The specific objectives of the thesis is to develop a method for breaking eggshell prior to nucleic acid extraction and to design and optimize a qPCR method to quantify viable Ascaris eggs.