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Identification of the genetic elements involved in pediocin PD-1 synthesis = Identification des éléments génétiques impliqués dans la

Dumas, Stéphane ; Schmid, Sergio (Dir.)

Mémoire de diplôme HES : Haute Ecole d'Ingénierie, 2007.

Aim: Confirm the presence of pedA gene, known part of pediocin PD-1 operon, in one of Pediococcus damnosus plasmids. Construct a plasmid library using this isolated plasmid, digested with endonucleases and subcloned in pZErO-2 vector. Identify recombinant plasmids containing pedA gene by PCR screening or/and by hybridization with pedA probe. Sequence the insert and localize pedA region in order...