Université de Fribourg

Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes

Steinmetz, Lukas ; Bourquin, Joel ; Barosova, Hana ; Haeni, Laetitia ; Caldwell, Jessica ; Milosevic, Ana ; Geers, Christoph ; Bonmarin, Mathias ; Taladriz-Blanco, Patricia ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Nanoscale, 2020, vol. 12, no. 33, p. 17362–17372

Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluorescent dyes. Here, we explore lock-in...

Université de Fribourg

A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures

Septiadi, Dedy ; Bourquin, Joel ; Durantie, Estelle ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Scientific Reports, 2018, vol. 8, no. 1, p. 9861

Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor...

Université de Fribourg

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

Durantie, Estelle ; Vanhecke, Dimitri ; Rodriguez-Lorenzo, Laura ; Delhaes, Flavien ; Balog, Sandor ; Septiadi, Dedy ; Bourquin, Joel ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Particle and Fibre Toxicology, 2017, vol. 14, p. 49

The lung represents the primary entry route for airborne particles into the human body. Most studies addressed possible adverse effects using single (nano)particles, but aerosolic nanoparticles (NPs) tend to aggregate and form structures of several hundreds nm in diameter, changing the physico-chemical properties and interaction with cells. Our aim was to investigate how aggregation might...