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Université de Fribourg

FRI-2 carbapenemase-producing Enterobacter cloacae complex in the UK

Danièle Meunier ; Findlay, Jacqueline ; Doumith, Michel ; Godoy, Daniel ; Perry, Claire ; Pike, Rachel ; Gronthoud, Firza ; Shryane, Theresa ; Poirel, Laurent ; Welfare, William ; Woodford, Neil ; Hopkins, Katie L.

In: Journal of Antimicrobial Chemotherapy, 2017, vol. 72, no. 9, p. 2478–2482

Objectives: Detection of rarer carbapenemases is challenging, as it requires molecular assays with comprehensive coverage or the use of phenotypic methods for the detection of carbapenemase activity. We describe a new class A carbapenemase, FRI-2, in an Enterobacter cloacae complex isolate following implementation of an in- house multiplex PCR for the detection of ‘rare’ class A...

Université de Fribourg

Key features of mcr-1-bearing plasmids from Escherichia coli isolated from humans and food

Zurfluh, Katrin ; Nüesch-Inderbinen, Magdalena ; Klumpp, Jochen ; Poirel, Laurent ; Nordmann, Patrice ; Stephan, Roger

In: Antimicrobial Resistance & Infection Control, 2017, vol. 6, p. 91

Mcr-1-harboring Enterobacteriaceae are reported worldwide since their first discovery in 2015. However, a limited number of studies are available that compared full-length plasmid sequences of human and animal origins.

Université de Fribourg

High-level resistance to colistin mediated by various mutations in the crrb gene among carbapenemase-producing klebsiella pneumoniae

Jayol, Aurélie ; Nordmann, Patrice ; Brink, Adrian ; Villegas, Maria-Virginia ; Dubois, Véronique ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2017, vol. 61, no. 11, p. e01423-17

Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin- resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB...

Université de Fribourg

CHROMagar mSuperCARBA and RAPIDEC® Carba NP test for detection of carbapenemase-producing Enterobacteriaceae

Garcia-Quintanilla, Meritxell ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2017, p. -

A novel chromogenic medium CHROMagar mSuperCARBA was evaluated to detect carbapenem-resistant Gram-negatives. This medium is as sensitive and as specific as the SUPERCARBA medium for detecting KPC, MBL and OXA-48-type producers (100% and 100%, respectively) and is compatible with subsequent testing of carbapenemase activity using the RAPIDEC® CARBA NP.

Université de Fribourg

Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

Alvarez-Marin, R. ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Kieffer, Nicolas ; Poirel, Laurent

In: European Journal of Clinical Microbiology & Infectious Diseases, 2017, p. 1–5

Multidrug-resistant (MR) Gram-negative (GN) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. Well-tolerated antiseptics, such as octenidine dihydrochloride (OCT), may be a very useful tool in infection control to reduce the dissemination of MRGN. This study aimed to investigate the...

Université de Fribourg

Recent advances in biochemical and molecular diagnostics for the rapid detection of antibiotic-resistant Enterobacteriaceae: a focus on ß-lactam resistance

Decousser, Jean-Winoc ; Poirel, Laurent ; Nordmann, Patrice

In: Expert Review of Molecular Diagnostics, 2017, vol. 17, no. 4, p. 327–350

The rapid detection of resistance is a challenge for clinical microbiologists who wish to prevent deleterious individual and collective consequences such as (i) delaying efficient antibiotic therapy, which worsens the survival rate of the most severely ill patients, or (ii) delaying the isolation of the carriers of multidrug-resistant bacteria and promoting outbreaks; this last consequence is...

Université de Fribourg

Plazomicin activity against polymyxin-resistant Enterobacteriaceae, including MCR-1-producing isolates

Denervaud-Tendon, Valérie ; Poirel, Laurent ; Connolly, Lynn E. ; Krause, Kevin M. ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2017, p. -

Objectives: Plazomicin, a novel aminoglycoside with in vitro activity against MDR Gram-negative organisms, is under development to treat patients with serious enterobacterial infections. We evaluated the activity of plazomicin and comparators against colistin-resistant enterobacterial isolates.Methods: Susceptibility to plazomicin and comparators was tested by broth microdilution for a collection...