Université de Fribourg

Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry

Chen, Jun ; Young, Susan M. ; Allen, Chris ; Seeber, Andrew ; Péli-Gulli, Marie-Pierre ; Panchaud, Nicolas ; Waller, Anna ; Ursu, Oleg ; Yao, Tuanli ; Golden, Jennifer E. ; Strouse, J. Jacob ; Carter, Mark B. ; Kang, Huining ; Bologa, Cristian G. ; Foutz, Terry D. ; Edwards, Bruce S. ; Peterson, Blake R. ; Aubé, Jeffrey ; Werner-Washburne, Margaret ; Loewith, Robbie J. ; De Virgilio, Claudio ; Sklar, Larry A.

In: ACS Chemical Biology, 2012, vol. 7, no. 4, p. 715–722

TOR (target of rapamycin) is a serine/threonine kinase, evolutionarily conserved from yeast to human, which functions as a fundamental controller of cell growth. The moderate clinical benefit of rapamycin in mTOR-based therapy of many cancers favors the development of new TOR inhibitors. Here we report a high-throughput flow cytometry multiplexed screen using five GFP-tagged yeast clones that...