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MCR-2-mediated plasmid-borne polymyxin resistance most likely originates from Moraxella pluranimalium

Poirel, Laurent ; Kieffer, Nicolas ; Fernandez-Garayzabal, José F. ; Vela, Ana Isabel ; Larpin, Yu ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2017, vol. 72, no. 10, p. 2947-2949

In vitro evaluation of dual carbapenem combinations against carbapenemase-producing Enterobacteriaceae

Poirel, Laurent ; Kieffer, Nicolas ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2016, vol. 71, no. 1, p. 156-161

Eradication of a multidrug-resistant, carbapenemase-producing Klebsiella pneumoniae isolate following oral and intra-rectal therapy with a custom made, lytic bacteriophage preparation

Corbellino, Mario ; Kieffer, Nicolas ; Kutateladze, Mzia ; Balarjishvili, Nana ; Leshkasheli, Lika ; Askilashvili, Lia ; Tsertsvadze, George ; Rimoldi, Sara Giordana ; Nizharadze, Deia ; Hoyle, Naomi ; Nadareishvili, Lia ; Antinori, Spinello ; Pagani, Cristina ; Scorza, Daniele Giuseppe ; Romanò, Ai Ling Loredana ; Ardizzone, Sandro ; Danelli, Piergiorgio ; Gismondo, Maria Rita ; Galli, Massimo ; Nordmann, Patrice ; Poirel, Laurent

In: Clinical Infectious Diseases, 2020, vol. 70, no. 9, p. 1998–2001

In July 2017, a patient presented colonization with a multidrug-resistant, carbapenemase (KPC-3)-producing Klebsiella pneumoniae isolate. A custom-made, lytic bacteriophage preparation was administered to the patient in December 2017, with subsequent eradication of the microorganism and without adverse effects.

ISEcp1 mediated transposition leads to fosfomycin and broad-spectrum cephalosporin resistance in Klebsiella pneumoniae

Kieffer, Nicolas ; Poirel, Laurent ; Mueller, Linda ; Mancini, Stefano ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 5, p. -

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed ISEcp1-blaCTX-M-14b tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the...

Characterization of FosL1, a plasmid-encoded fosfomycin resistance protein identified in Escherichia coli

Kieffer, Nicolas ; Poirel, Laurent ; Descombes, Marie-Christine ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 4, p. -

Fosfomycin is gaining renewed interest for treating urinary tract infections. Monitoring fosfomycin resistance is therefore important in order to detect the emergence of novel resistance mechanisms. Here, we used the Rapid Fosfomycin NP test to screen a collection of extended-spectrum-β-lactamase- producing Escherichia coli isolates from Switzerland and found a fosfomycin- resistant isolate...

In-vitro evaluation of a dual carbapenem combination against carbapenemase-producing Acinetobacter baumannii

Nordmann, Patrice ; Perler, Julien ; Kieffer, Nicolas ; Poirel, Laurent

In: Journal of Infection, 2020, vol. 80, no. 1, p. 121–142

Increased resistance to carbapenems in proteus mirabilis mediated by amplification of the bla vim-1 -carrying and is 26 -associated class 1 integron

Bontron, Séverine ; Poirel, Laurent ; Kieffer, Nicolas ; Savov, Encho ; Trifonova, Angelina ; Todorova, Iva ; Kueffer, Gwendoline ; Nordmann, Patrice

In: Microbial Drug Resistance, 2019, vol. 25, no. 5, p. 663–667

The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates.Methods: The entire genetic structure surrounding the β- lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing.Results: A series of clinical P. mirabilis isolates were consecutively ...

The inoculum effect of Escherichia coli expressing mcr-1 or not on colistin activity in a murine model of peritonitis

Fantin, B. ; Poujade, J. ; Grégoire, N. ; Chau, F. ; Roujansky, A. ; Kieffer, Nicolas ; Berleur, M. ; Couet, W. ; Nordmann, Patrice

In: Clinical Microbiology and Infection, 2019, vol. 25, no. 12, p. 1563.e5-1563.e8

Colistin often remains the last resort antibiotic active against carbapenemase- producing Enterobacteriaceae. However, while in vitro inoculum effect has been reported, therapeutic relevance of this phenomenon remains questioned.Methods: Ten E. coli strains were used that included the wild-type CFT073 and its transconjugant CFT073-MCR-1 and eight susceptible clinical isolates. Mice with ...

Identification of fosa8, a plasmid-encoded fosfomycin resistance determinant from Escherichia coli, and its origin in Leclercia adecarboxylata

Poirel, Laurent ; Vuillemin, Xavier ; Kieffer, Nicolas ; Mueller, Linda ; Descombes, Marie-Christine ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 11, p. -

A plasmid-located fosfomycin resistance gene, fosA8, was identified from a CTX-M- 15-producing Escherichia coli isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The fosA8 gene was located on a transferable 50-kb...

ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans

Kieffer, Nicolas ; Guzmán-Puche, Julia ; Poirel, Laurent ; Kang, Hyo Jung ; Jeon, Che Ok ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1568–1571

Objectives: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment.Methods: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme.Results: The enzyme named ZHO-1...