Université de Neuchâtel

Isolation and characterization of oxalotrophic bacteria from tropical soils

Bravo, Daniel ; Braissant, Olivier ; Cailleau, Guillaume ; Verrecchia, Eric ; Junier, Pilar

In: Archives of Microbiology, 2015, vol. 197, no. 1, p. 65-77

The oxalate–carbonate pathway (OCP) is a biogeochemical set of reactions that involves the conversion of atmospheric CO2 fixed by plants into biomass and, after the biological recycling of calcium oxalate by fungi and bacteria, into calcium carbonate in terrestrial environments. Oxalotrophic bacteria are a key element of this process because of their ability to oxidize calcium...

Université de Neuchâtel

Metaproteomics and ultrastructure characterization of Komagataeibacter spp. involved in high-acid spirit vinegar production

Andrés-Barrao, Cristina ; Saad, Maged M ; Cabello Ferrete, Elena ; Bravo, Daniel ; Chappuis, Marie-Luise ; Ortega Pérez, Ruben ; Junier, Pilar ; Perret, Xavier ; Barja, François

In: Food Microbiology, 2016, vol. 55, p. 112-122

Acetic acid bacteria (AAB) are widespread microorganisms in nature, extensively used in food industry to transform alcohols and sugar alcohols into their corresponding organic acids. Specialized strains are used in the production of vinegar through the oxidative transformation of ethanol into acetic acid. The main AAB involved in the production of high-acid vinegars using the submerged...

Université de Neuchâtel

Assessing the diversity and metabolism of oxalotrophic bacteria in tropical soils

Bravo, Daniel ; Junier, Pilar (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2013.

Climate change is increasing as a consequence of elevated concentrations of CO2 in the atmosphere. Among the scientific strategies proposed to tackle the effect of elevated CO2, the biogeochemical oxalate-carbonate pathway (OCP) occurring in terrestrial habitats, appears to be important since it is considered as a potential carbon sink. This process occurs naturally in...

Université de Neuchâtel

Fungi, bacteria and soil pH : the oxalate–carbonate pathway as a model for metabolic interaction

Martin, Gaëtan ; Guggiari, Matteo ; Bravo, Daniel ; Zopfi, Jakob ; Cailleau, Guillaume ; Aragno, Michel ; Job, Daniel ; Verrecchia, Eric ; Junier, Pilar

In: Environmental Microbiology, 2012, vol. 14, no. 11, p. 2960-2970

The oxalate–carbonate pathway involves the oxidation of calcium oxalate to low-magnesium calcite and represents a potential long-term terrestrial sink for atmospheric CO2. In this pathway, bacterial oxalate degradation is associated with a strong local alkalinization and subsequent carbonate precipitation. In order to test whether this process occurs in soil, the role of bacteria,...

Université de Neuchâtel

Use of an isothermal microcalorimetry assay to characterize microbial oxalotrophic activity

Bravo, Daniel ; Braissant, Olivier ; Solokhina, Anna ; Clerc, Martin ; Daniels, Alma U. ; Verrecchia, Eric ; Junier, Pilar

In: FEMS Microbiology Ecology, 2011, vol. 78, no. 2, p. 266–274

Isothermal microcalorimetry (IMC) has been used in the past to monitor metabolic activities in living systems. A few studies have used it on ecological research. In this study, IMC was used to monitor oxalotrophic activity, a widespread bacterial metabolism found in the environment, and particularly in soils. Six model strains were inoculated in solid angle media with K-oxalate as the sole carbon...