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Selective purification of recombinant neuroactive peptides using the flagellar type III secretion system

Singer, Hanna M. ; Erhardt, Marc ; Steiner, Andrew M. ; Zhang, Min-Min ; Yoshikami, Doju ; Bulaj, Grzegorz ; Olivera, Baldomero M. ; Hughes, Kelly T.

In: mBio, 2012, vol. 3, no. 3, p. e00115-12t

The structure, assembly, and function of the bacterial flagellum involves about 60 different proteins, many of which are selectively secreted via a specific type III secretion system (T3SS) (J. Frye et al., J. Bacteriol. 188:2233–2243, 2006). The T3SS is reported to secrete proteins at rates of up to 10,000 amino acid residues per second. In this work, we showed that the flagellar T3SS of...