Université de Fribourg

The Caenorhabditis elegans LET-418/Mi2 plays a conserved role in lifespan regulation

Vaux, Véronique De ; Pfefferli, Catherine ; Passannante, Myriam ; Belhaj, Khaoula ; Essen, Alina von ; Sprecher, Simon G. ; Müller, Fritz ; Wicky, Chantal

In: Aging Cell, 2013, p. –

The evolutionarily conserved nucleosome-remodeling protein Mi2 is involved in transcriptional repression during development in various model systems, plays a role in embryonic patterning and germ line development, and participates in DNA repair and cell cycle progression. It is the catalytic subunit of the nucleosome remodeling and histone deacetylase (NuRD) complex, a key determinant of...

Université de Fribourg

Different Mi-2 Complexes for Various Developmental Functions in Caenorhabditis elegans

Passannante, Myriam ; Marti, Claude-Olivier ; Pfefferli, Catherine ; Moroni, Paolo S. ; Kaeser-Pebernard, Stéphanie ; Puoti, Alessandro ; Hunziker, Peter ; Wicky, Chantal ; Müller, Fritz

In: PLoS ONE, 2010, vol. 5, no. 10, p. e13681

Biochemical purifications from mammalian cells and Xenopus oocytes revealed that vertebrate Mi-2 proteins reside in multisubunit NuRD (Nucleosome Remodeling and Deacetylase) complexes. Since all NuRD subunits are highly conserved in the genomes of C. elegans and Drosophila, it was suggested that NuRD complexes also exist in invertebrates. Recently, a novel dMec complex, composed of dMi-2 and...

Université de Fribourg

The C. elegans sex determination protein MOG-3 functions in meiosis and binds to the CSL co-repressor CIR-1

Kasturi, Prasad ; Zanetti, Simone ; Passannante, Myriam ; Saudan, Zarifja ; Müller, Fritz ; Puoti, Alessandro

In: Developmental Biology, 2010, vol. 344, no. 2, p. 593-602

In the germ line of the Caenorhabditis elegans hermaphrodite, nuclei either proliferate through mitosis or initiate meiosis, finally differentiating as spermatids or oocytes. The production of oocytes requires repression of the fem-3 mRNA by cytoplasmic FBF and nuclear MOG proteins. Here we report the identification of the sex determining gene mog-3 and show that in addition to its role in gamete...

Université de Fribourg

Multiple genetic pathways involving the Caenorhabditis elegans Bloom's syndrome genes him-6, rad-51, and top-3 are needed to maintain genome stability in the germ line

Wicky, Chantal ; Alpi, Arno ; Passannante, Myriam ; Rose, Ann ; Gartner, Anton ; Müller, Fritz

In: Molecular and Cellular Biology, 2004, vol. 24, p. 5016-5027

Bloom's syndrome (BS) is an autosomal-recessive human disorder caused by mutations in the BS RecQ helicase and is associated with loss of genomic integrity and an increased incidence of cancer. We analyzed the mitotic and the meiotic roles of Caenorhabditis elegans him-6, which we show to encode the ortholog of the human BS gene. Mutations in him-6 result in an enhanced irradiation sensitivity, a...