Université de Fribourg

Phase transformation of superparamagnetic iron oxide nanoparticles via thermal annealing: implications for hyperthermia applications

Crippa, Federica ; Rodriguez-Lorenzo, Laura ; Hua, Xiao ; Goris, Bart ; Bals, Sara ; Garitaonandia, José S. ; Balog, Sandor ; Burnand, David ; Hirt, Ann M. ; Haeni, Laetitia ; Lattuada, Marco ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: ACS Applied Nano Materials, 2019, vol. 2, no. 7, p. 4462–4470

Magnetic hyperthermia has the potential to play an important role in cancer therapy and its efficacy relies on the nanomaterials selected. Superparamagnetic iron oxide nanoparticles (SPIONs) are excellent candidates due to the ability of producing enough heat to kill tumor cells by thermal ablation. However, their heating properties depend strongly on crystalline structure and size, which may...

Université de Fribourg

Polymer-coated gold nanospheres do not impair the innate immune function of human b lymphocytes in vitro

Hočevar, Sandra ; Milošević, Ana ; Rodriguez-Lorenzo, Laura ; Ackermann-Hirschi, Liliane ; Mottas, Ines ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Bourquin, Carole ; Clift, Martin James David

In: ACS Nano, 2019, vol. 13, no. 6, p. 6790–6800

Gold nanoparticles (GNPs) are intended for use within a variety of biomedical applications due to their physicochemical properties. Although, in general, biocompatibility of GNPs with immune cells such as macrophages and dendritic cells is well established, the impact of GNPs on B lymphocyte immune function remains to be determined. Since B lymphocytes play an important role in health and...

Université de Fribourg

A hydrofluoric acid-free method to dissolve and quantify silica nanoparticles in aqueous and solid matrices

Bossert, David ; Urban, Dominic A. ; Maceroni, Mattia ; Ackermann-Hirschi, Liliane ; Haeni, Laetitia ; Yajan, Phattadon ; Spuch-Calvar, Miguel ; Rothen-Rutishauser, Barbara ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Schwab, Fabienne

In: Scientific Reports, 2019, vol. 9, no. 1, p. 7938

As the commercial use of synthetic amorphous silica nanomaterials (SiO2-NPs) increases, their effects on the environment and human health have still not been explored in detail. An often-insurmountable obstacle for SiO2-NP fate and hazard research is the challenging analytics of solid particulate silica species, which involves toxic and corrosive hydrofluoric acid (HF). We therefore developed...

Université de Fribourg

Artificial lysosomal platform to study nanoparticle long-term stability

Milosevic, Ana ; Bourquin, Joël ; Burnand, David ; Lemal, Philipp ; Crippa, Federica ; Monnier, Christophe A. ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: CHIMIA International Journal for Chemistry, 2019, vol. 73, no. 1, p. 55–58

Nanoparticles (NPs) possess unique properties useful for designing specific functionalities for biomedi- cal applications. A prerequisite of a safe-by-design and effective use in any biomedical application is to study NP–cell interactions to gain a better understanding of cellular consequences upon exposure. Cellular uptake of NPs results mainly in the localization of NPs in the complex...

Université de Fribourg

Nanoparticle administration method in cell culture alters particle-cell interaction

Moore, Thomas L. ; Urban, Dominic A. ; Rodriguez-Lorenzo, Laura ; Milosevic, Ana ; Crippa, Federica ; Spuch-Calvar, Miguel ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Lattuada, Marco ; Petri-Fink, Alke

In: Scientific Reports, 2019, vol. 9, no. 1, p. 900

As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano- characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how in vitro...

Université de Fribourg

A rational and iterative process for targeted nanoparticle design and validation

Rodriguez-Lorenzo, Laura ; D.Rafiee, Sarah ; Reis, Corine ; Milosevic, Ana ; L.Moore, Thomas ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Rüegg, Curzio ; Petri-Fink, Alke

In: Colloids and Surfaces B: Biointerfaces, 2018, vol. 171, p. 579–589

The lack of understanding of fundamental nano-bio interactions, and difficulties in designing particles stable in complex biological environments are major limitations to their translation into biomedical clinical applications. Here we present a multi- parametric approach to fully characterize targeted nanoparticles, and emphasizes the significant effect that each detail in the synthetic...

Université de Fribourg

Carbon nanodots: Opportunities and limitations to study their biodistribution at the human lung epithelial tissue barrier

Durantie, Estelle ; Barosova, Hana ; Drasler, Barbara ; Rodriguez-Lorenzo, Laura ; Urban, Dominic A. ; Vanhecke, Dimitri ; Septiadi, Dedy ; Hirschi-Ackermann, Liliane ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Biointerphases, 2018, vol. 13, no. 6, p. 06D404

Inhalation of combustion-derived ultrafine particles (≤0.1 μm) has been found to be associated with pulmonary and cardiovascular diseases. However, correlation of the physicochemical properties of carbon-based particles such as surface charge and agglomeration state with adverse health effects has not yet been established, mainly due to limitations related to the detection of carbon...

Université de Fribourg

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

Chortarea, Savvina ; Fytianos, Kleanthis ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine, 2018, vol. 13, no. 10, p. 1169–1185

Aim: The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH2) upon repeated administration onto a 3D lung model were explored. Materials & methods: AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission ...

Université de Fribourg

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

Durantie, Estelle ; Vanhecke, Dimitri ; Rodriguez-Lorenzo, Laura ; Delhaes, Flavien ; Balog, Sandor ; Septiadi, Dedy ; Bourquin, Joel ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Particle and Fibre Toxicology, 2017, vol. 14, p. 49

The lung represents the primary entry route for airborne particles into the human body. Most studies addressed possible adverse effects using single (nano)particles, but aerosolic nanoparticles (NPs) tend to aggregate and form structures of several hundreds nm in diameter, changing the physico-chemical properties and interaction with cells. Our aim was to investigate how aggregation might...

Université de Fribourg

Quantifying nanoparticle cellular uptake: which method is best?

Drasler, Barbara ; Vanhecke, Dimitri ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine, 2017, vol. 12, no. 10, p. 1095–1099

As the range of engineered nanoparticles (NPs) designed as specific carriers increases, for example for cell targeting and drug delivery, the question on how many NPs are interacting or are taken up by cells is becoming increasingly important for any potential biomedical application. On one hand, the delivered dose of such NPs to the targeted cells is a key parameter in the assessment of...