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Statistical significance of quantitative PCR

Karlen, Yann ; McNair, Alan ; Perseguers, Sébastien ; Mazza, Christian ; Mermod, Nicolas

In: BMC Bioinformatics, 2007, vol. 8, p. 131

Background: PCR has the potential to detect and precisely quantify specific DNA sequences, but it is not yet often used as a fully quantitative method. A number of data collection and processing strategies have been described for the implementation of quantitative PCR. However, they can be experimentally cumbersome, their relative performances have not been evaluated systematically, and they...