Université de Fribourg

VGLUT1 binding to endophilin or intersectin1 and dynamin phosphorylation in a diurnal context

Richter, Karin ; Schmutz, Isabelle ; Darna, Mahesh ; Zander, Johannes-F ; Chavan, Rohit ; Albrecht, Urs ; Ahnert-Hilger, Gudrun

In: Neuroscience, 2018, vol. 371, p. 29–37

Glutamate is concentrated into synaptic vesicles (SV) by the vesicular glutamate transporters (VGLUT) 1 and 2. VGLUTs also harbor a Na+/Pi-transport activity when residing at the plasma membrane. Here we aimed to identify whether the diurnal switches of VGLUT1 parallels interactions with or modification of endocytic proteins.VGLUT1 and dynamin bind to SH3 domains of either endophilin (Enph)...

Université de Fribourg

Differential Sorting of the Vesicular Glutamate Transporter 1 into a Defined Vesicular Pool Is Regulated by Light Signaling Involving the Clock Gene Period2

Yelamanchili, Sowmya V. ; Pendyala, Gurudutt ; Brunk, Irene ; Darna, Mahesh ; Albrecht, Urs ; Ahnert-Hilger, Gudrun

In: The Journal of Biological Chemistry, 2006, vol. 281, no. 23, p. 15671–15679

Synaptic strength depends on the amount of neurotransmitter stored in synaptic vesicles. The vesicular transmitter content has recently been shown to be directly dependent on the expression levels of vesicular neurotransmitter transporters indicating that the transport capacity of synaptic vesicles is a critical determinant for synaptic efficacy. Using synaptic vesicles prepared from whole brain...