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    Université de Fribourg

    An analog to digital converter controls bistable transfer competence development of a widespread bacterial integrative and conjugative element

    Carraro, Nicolas ; Richard, Xavier ; Sulser, Sandra ; Delavat, Franc¸ois ; Mazza, Christian ; van der Meer, Jan Roelof

    In: eLife, 2020, vol. 9, p. e57915

    Conjugative transfer of the integrative and conjugative element ICEclc in Pseudomonas requires development of a transfer competence state in stationary phase, which arises only in 3–5% of individual cells. The mechanisms controlling this bistable switch between non-active and transfer competent cells have long remained enigmatic. Using a variety of genetic tools and epistasis experiments in...

    Université de Fribourg

    Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips: Chemotaxis-microfluidic biosensor

    Roggo, Clémence ; Picioreanu, Cristian ; Richard, Xavier ; Mazza, Christian ; Lintel, Harald van ; Meer, Jan Roelof van der

    In: Environmental Microbiology, 2018, vol. 20, no. 1, p. 241–258

    Whole-cell bacterial bioreporters are proposed as alternatives to chemical analysis of, for example, pollutants in environmental compartments. Commonly based on reporter gene induction, bioreporters produce a detectable signal within 30 min to a few hours after exposure to the chemical target, which is impractical for applications aiming at a fast response. In an attempt to attain faster...