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Université de Neuchâtel

The Internal Propeptide of the Ricin Precursor Carries a Sequence-Specific Determinant for Vacuolar Sorting

Frigerio, Lorenzo ; Jolliffe, Nicholas A. ; Di Cola, Alessandra ; Hernández Felipe, Doramys ; Paris, Nadine ; Neuhaus, Jean-Marc ; Lord, J. Michael ; Ceriotti, Aldo ; Roberts, Lynne M.

In: Plant Physiology, 2001, vol. 126, p. 167-175

Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linker is removed. Here, we replicate these events in transfected tobacco (Nicotiana...

Université de Neuchâtel

The C-terminal tetrapeptide of phaseolin is sufficient to target green fluorescent protein to the vacuole

Frigerio, Lorenzo ; Foresti, Ombretta ; Hernández Felipe, Doramys ; Neuhaus, Jean-Marc ; Vitale, Alessandro

In: Journal of Plant Physiology, 2001, vol. 158, p. 499-503

Phaseolin is a vacuolar storage glycoprotein synthesized as a precursor with a short C-terminal propeptide. We have recently shown that deletion of the last four C-terminal amino acids (AFVY, which are part of, or constitute the propeptide) abolishes vacuolar targeting, causing phaseolin to be secreted. Here we provide biochemical and microscopical evidence that the AFVY tetrapeptide, when fused...

Université de Neuchâtel

Evaluation of a yeast system for studying the function of plant vacuolar sorting receptors (VSRs)

Hodel Hernandez, Doramys ; Neuhaus, Jean-Marc (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2005 ; 1800.

The yeast S.cerevisiae is not an efficient tool for in vivo studies of plant vacuolar sorting receptors Plant and yeast vacuoles, the equivalents of mammalian lysosomes, are acidic compartments involved in hydrolytic functions. They are also essential for metabolite storage and for maintaining cytosolic ion and pH homeostasis (Klionsky and Emr, 1990; Wink, 1993). The vacuoles mainly receive...

Université de Neuchâtel

Bacterial community associated with the rhizosphere of wheat: interactions with arbuscular mycorrhizal fungi and selection of plant growth promoting rhizobacteria for the increase of wheat growth and soil health in Indian marginal rainfed fields

Roesti, David ; Aragno, Michel (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2005 ; 1790.

The objective of this thesis was to improve our knowledge on the interactions between wheat, rhizobacteria and arbuscular mycorrhizal fungi (AMF) in the mycorrhizosphere in order to define criteria for the selection of plant growth promoting bacteria (PGPR) strains in view of a PGPR/AMF dual inoculation in Indian wheat fields. First, microcosm systems were set-up to obtain mycorrhizosphere,...

Université de Neuchâtel

Typologie et dynamique de la végétation des zones alluviales de Suisse

Roulier, Christian ; Gobat, Jean-Michel (Dir.)

Thèse de doctorat : Université de Neuchâtel, 1997 ; 1405.

En suisse, l'inventaire fédéral des zones alluviales d'importance nationale contient 169 objets situés entre 195 et 2210 m d'altitude. Ces sites sont protégés par une ordonnance fédérale en vigueur depuis 1992. Certains d'entre eux présentent une dynamique naturelle liée aux inondations et au rejeunissement par les crues. D'autres ont subi de profondes modifications suite à des...

Université de Neuchâtel

Advances in biological tracer techniques for hydrology and hydrogeology using bacteriophages : Optimization of the methods and investigation of the behavior of bacterial viruses in surface waters and in porous and fractured aquifers = Utilisation de bactériophages comme traceurs en hydrologie et en hydrogéologie

Rossi, Pierre ; Aragno, Michel (Dir.) ; Muller, Imre (Codir.)

Thèse de doctorat : Université de Neuchâtel : 1994 ; 1570.

This work was undertaken in the Laboratoire de Microbiologie of the University of Neuchâtel, in close collaboration with the Center of Hydrogeology (CHYN). This multi-disciplinary research project was instigated because of the growing need both for environmentally harmless hydrogeological investigation tools, and for new tracers that can be used in multitracing experiments. This work treats of...

Université de Neuchâtel

A physiological and molecular approach to study organic acid exudation and development of cluster roots in Lupinus albus L

Langlade, Nicolas Bernard ; Martinoia, E (Dir.)

Thèse de doctorat : Université de Neuchâtel : 2002 ; 1614.

Cluster roots (or proteoid roots) are bottlebrush-like structures, developed by hundreds of plant species (mainly of Proteaceae family, but also the legume Lupinus albus L.) induced by nutrient stresses, especially P. These structures are characterized by a strong capacity to exude citrate (up to 7 µmol .h-1 .g-1) and malate in the rhizosphere and thus to increase the availability of P, Fe and...