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Université de Fribourg

Effect of 3-mercaptopyruvate sulfurtransferase deficiency on the development of multiorgan failure, inflammation, and wound healing in mice subjected to burn injury

Ahmad, Akbar ; Druzhyna, Nadiya ; Szabo, Csaba

In: Journal of Burn Care & Research, 2019, vol. 40, no. 2, p. 148–156

The gaseous transmitter hydrogen sulfide (H2S) has been implicated in various forms of critical illness. Here, we have compared the outcome of scald burn injury in wild- type mice and in mice deficient in 3-mercaptopyruvate sulfurtransferase (3-MST), a mammalian H2S-generating enzyme. Outcome variables included indices of organ injury, clinical chemistry parameters, and plasma levels of...

Université de Fribourg

Initial viral inoculum determines kinapse-and synapse-like t cell motility in reactive lymph nodes

Sivapatham, Sujana ; Ficht, Xenia ; Albuquerque, Juliana Barreto de ; Page, Nicolas ; Merkler, Doron ; Stein, Jens V.

In: Frontiers in Immunology, 2019, vol. 10, p. -

T cell activation in lymphoid tissue occurs through interactions with cognate peptide- major histocompatibility complex (pMHC)-presenting dendritic cells (DCs). Intravital imaging studies using ex vivo peptide-pulsed DCs have uncovered that cognate pMHC levels imprint a wide range of dynamic contacts between these two cell types. T cell—DC interactions vary between transient,...

Université de Fribourg

Are integrins still practicable targets for anti-cancer therapy?

Alday-Parejo, Begoña ; Stupp, Roger ; Rüegg, Curzio

In: Cancers, 2019, vol. 11, no. 7, p. 978

Correlative clinical evidence and experimental observations indicate that integrin adhesion receptors, in particular those of the αV family, are relevant to cancer cell features, including proliferation, survival, migration, invasion, and metastasis. In addition, integrins promote events in the tumor microenvironment that are critical for tumor progression and metastasis, including tumor...

Université de Fribourg

Cooccurrence of NDM-1, ESBL, RmtC, AAC(6′)-Ib, and QnrB in Clonally Related Klebsiella pneumoniae Isolates Together with Coexistence of CMY-4 and AAC(6′)-Ib in Enterobacter cloacae Isolates from Saudi Arabia

Al-Agamy, Mohamed H. ; El-Mahdy, Taghrid S. ; Radwan, Hesham H. ; Poirel, Laurent

In: BioMed Research International, 2019, p. -

The aim of this study was to investigate the mechanisms responsible for resistance to antimicrobials in a collection of enterobacterial isolates recovered from two hospitals in Saudi Arabia. A total of six strains isolated from different patients showing high resistance to carbapenems was recovered in 2015 from two different hospitals, with four being Klebsiella pneumoniae and two...

Université de Fribourg

ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans

Kieffer, Nicolas ; Guzmán-Puche, Julia ; Poirel, Laurent ; Kang, Hyo Jung ; Jeon, Che Ok ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1568–1571

Objectives: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment.Methods: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme.Results: The enzyme named ZHO-1...

Université de Fribourg

A selective culture medium for screening linezolid-resistant gram-positive bacteria

Nordmann, Patrice ; Rodríguez-Villodres, Angel ; Poirel, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2019, vol. 95, no. 1, p. 1–4

The SuperLinezolid medium was developed for screening resistance to linezolid (LZD) in Gram-positive bacteria (Staphylococcus spp., Enterococcus spp.). It was evaluated using LZD-susceptible (n = 20) and LZD-resistant (n = 17) Gram-positive isolates. The sensitivity was found to be 82% at 24 h (3 out of 17 isolates being missed), and reached 100% at 48 h. At 48 h, a single...

Université de Fribourg

mcr-9, An inducible gene encoding an acquired phosphoethanolamine transferase in Escherichia coli, and its origin

Kieffer, Nicolas ; Royer, b Guilhem ; Decousser, Jean-Winoc ; Bourrel, Anne-Sophie ; Palmieri, Mattia ; Ortiz De La Rosa, Jose-Manuel ; Jacquier, Hervé ; Denamur, Erick ; Nordmann, Patrice ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 9, p. -

The plasmid-located mcr-9 gene, encoding a putative phosphoethanolamine transferase, was identified in a colistin-resistant human fecal Escherichia coli strain belonging to a very rare phylogroup, the D-ST69-O15:H6 clone. This MCR-9 protein shares 33% to 65% identity with the other plasmid-encoded MCR-type enzymes identified (MCR-1 to -8) that have been found as sources of acquired resistance...

Université de Fribourg

Epidemiology of Carbapenemase-Producing Klebsiella pneumoniae in a Hospital, Portugal

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Gonçalves, Maria Luísa ; Pereira, Ana Luísa ; Nordmann, Patrice ; Poirel, Laurent

In: Emerging Infectious Diseases, 2019, vol. 25, no. 9, p. 1632–1638

We aimed to provide updated epidemiologic data on carbapenem-resistant Klebsiella pneumoniae in Portugal by characterizing all isolates (N = 46) recovered during 2013– 2018 in a 123-bed hospital in Lisbon. We identified blaKPC-3 (n = 36), blaOXA-181 (n = 9), and blaGES-5 (n = 8) carbapenemase genes and observed co-occurrence of blaKPC-3 and blaGES-5 in 7 isolates. A single GES-5–producing...

Université de Fribourg

Efficacy of colistin alone and in various combinations for the treatment of experimental osteomyelitis due to carbapenemase-producing Klebsiella pneumoniae

Crémieux, Anne-Claude ; Dinh, Aurélien ; Nordmann, Patrice ; Mouton, William ; Tattevin, Pierre ; Ghout, Idir ; Jayol, Aurelie ; Aimer, Omar ; Gatin, Laure ; Verdier, Marie-Clémence ; Saleh-Mghir, Azzam ; Laurent, Frédéric

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 9, p. 2666–2675

Objectives: In a new experimental model of carbapenemase-producing Klebsiella pneumoniae osteomyelitis we evaluated the efficacy of colistin alone and in various combinations and examined the emergence of colistin-resistant strains and cross- resistance to host defence peptides (HDPs).Methods: KPC-99YC is a clinical strain with intermediate susceptibility to meropenem (MIC = 4 mg/L) and...